Articles
A sesquiterpenes-rich fraction from potato with bioactive properties against selected leukemia cell lines
Article number
1329_7
Pages
41 – 50
Language
English
Abstract
Secondary metabolites play significant functions in plants.
Among the secondary metabolites are the phytoalexins, which are involved in plant defense against pathogenic microorganisms.
In the Solanaceae, such as potato, the known phytoalexins belong to the sesquiterpenes family.
In the present study, the sesquiterpene phytoalexins were induced in tubers from several breeding clones and commercial potato cultivars using a low ultraviolet dose.
The evaluated genotypes showed differences in their responses to treatment and could be categorized by their qualitative and quantitative sesquiterpene phytoalexins.
The potential bioactivity of a sesquiterpenes-rich extract prepared from a commercial potato cultivar was evaluated against 3 model cancer cell lines, namely A549 pulmonary carcinoma, DLD-1 colorectal adenocarcinoma and THP-1 monocytic leukemia.
The Alamar blue and the Annexin V assays were used respectively to measure the induction of cytotoxicity and of apoptotic cell death caused by the potato extract.
The phytoalexin resveratrol was used as a reference compound to compare the bioactivity of the extract from potato.
The data indicate that the sesquiterpenes-rich extract from potato possesses apoptotic activity against selected cancer cell lines.
Among the secondary metabolites are the phytoalexins, which are involved in plant defense against pathogenic microorganisms.
In the Solanaceae, such as potato, the known phytoalexins belong to the sesquiterpenes family.
In the present study, the sesquiterpene phytoalexins were induced in tubers from several breeding clones and commercial potato cultivars using a low ultraviolet dose.
The evaluated genotypes showed differences in their responses to treatment and could be categorized by their qualitative and quantitative sesquiterpene phytoalexins.
The potential bioactivity of a sesquiterpenes-rich extract prepared from a commercial potato cultivar was evaluated against 3 model cancer cell lines, namely A549 pulmonary carcinoma, DLD-1 colorectal adenocarcinoma and THP-1 monocytic leukemia.
The Alamar blue and the Annexin V assays were used respectively to measure the induction of cytotoxicity and of apoptotic cell death caused by the potato extract.
The phytoalexin resveratrol was used as a reference compound to compare the bioactivity of the extract from potato.
The data indicate that the sesquiterpenes-rich extract from potato possesses apoptotic activity against selected cancer cell lines.
Publication
Authors
M.T. Charles, D. Rolland, B. Bizimungu, F. Boudreau
Keywords
in vitro, monocytic leukemia, phytoalexins, rishitin, secondary metabolites, UV-C hormesis
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