Articles
Analytical methods for quantitation of amino acids from melons: a review
Article number
1329_9
Pages
65 – 76
Language
English
Abstract
Fruits and vegetables are rich in amino acids, which play significant roles in human health, specifically as the building blocks of proteins, precursors for nitric oxide (NO), and as neurotransmitters.
For example, the amino acid neurotransmitter γ-aminobutyric acid (GABA) prevents excitotoxicity, and NO acts as a vasodilator to improve cardiovascular health.
The chromatographic separation of amino acids is challenging due to their structural and physiochemical properties.
Therefore, a derivatization step to improve amino acid detection and chromatography resolution is warranted.
Moreover, the selection of chromatography parameters such as temperature, column, solvent acidity, and type of detector (ultraviolet, diode array, fluorescence, or mass spectrometry) are important criteria during analytical method development.
Plants of the Cucurbitaceae family are widely grown for their nutritional and medicinal attributes.
These vegetables serve as good sources of phytochemicals, including amino acids.
Therefore, the present review provides an overview of the analytical techniques applied for the determination of amino acids from melons such as Cucumis spp., Citrullus spp., and bitter melon (Momordica charantia).
For example, the amino acid neurotransmitter γ-aminobutyric acid (GABA) prevents excitotoxicity, and NO acts as a vasodilator to improve cardiovascular health.
The chromatographic separation of amino acids is challenging due to their structural and physiochemical properties.
Therefore, a derivatization step to improve amino acid detection and chromatography resolution is warranted.
Moreover, the selection of chromatography parameters such as temperature, column, solvent acidity, and type of detector (ultraviolet, diode array, fluorescence, or mass spectrometry) are important criteria during analytical method development.
Plants of the Cucurbitaceae family are widely grown for their nutritional and medicinal attributes.
These vegetables serve as good sources of phytochemicals, including amino acids.
Therefore, the present review provides an overview of the analytical techniques applied for the determination of amino acids from melons such as Cucumis spp., Citrullus spp., and bitter melon (Momordica charantia).
Publication
Authors
J. Singh, R. Metrani, G.K. Jayaprakasha, B.S. Patil
Keywords
amino acids, neurotransmitter, citrulline, melon, Cucurbitaceae
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