Articles
Large scale in vitro micropropagation of an ornamental plant, Oxalis triangularis A.st.-Hil, for commercial application
Article number
1339_31
Pages
245 – 256
Language
English
Abstract
Oxalis triangularis A.st.-Hil is an attractive ornamental plant propagated by bulbs and has no natural viable seeds.
In vitro micropropagation and acclimatization for the ornamental Oxalis triangularis A.st.- Hil, are reported.
Plantlet regeneration ability on MS medium supplemented with 6-benzylaminopurine (BAP) at different concentrations (0, 1.0, 3.0, and 5.0 mg L‑1) either singly or in combination with α-naphthalene acetic acid (NAA) (0.5 mg L‑1) or 2,4-dichlorophenoxy acetic acid (2,4-D) (0.5 mg L‑1) was evaluated using petiole explants.
Prolific shoot multiplication (28.40±0.12 shoots explant‑1), mean shoot length (28.94±0.14 mm), maximum number of roots (16.00±0.12 roots shoot‑1), and average root length (26.45±0.16 mm) was achieved on MS medium supplemented with 3.0 mg L‑1 BAP, and 0.5 mg L‑1 2,4-D. Micropropagated plantlets were successfully transferred in sterilized mixture of soil: vermiculite (3:1) with 100% survival rate under field conditions.
Regenerated plantlets were morphologically identical with mother plants.
This system can be used for rapid mass clonal propagation of Oxalis triangularis A.st.-Hil, for commercial production, conservation strategies, and producing phytomedicines.
In vitro micropropagation and acclimatization for the ornamental Oxalis triangularis A.st.- Hil, are reported.
Plantlet regeneration ability on MS medium supplemented with 6-benzylaminopurine (BAP) at different concentrations (0, 1.0, 3.0, and 5.0 mg L‑1) either singly or in combination with α-naphthalene acetic acid (NAA) (0.5 mg L‑1) or 2,4-dichlorophenoxy acetic acid (2,4-D) (0.5 mg L‑1) was evaluated using petiole explants.
Prolific shoot multiplication (28.40±0.12 shoots explant‑1), mean shoot length (28.94±0.14 mm), maximum number of roots (16.00±0.12 roots shoot‑1), and average root length (26.45±0.16 mm) was achieved on MS medium supplemented with 3.0 mg L‑1 BAP, and 0.5 mg L‑1 2,4-D. Micropropagated plantlets were successfully transferred in sterilized mixture of soil: vermiculite (3:1) with 100% survival rate under field conditions.
Regenerated plantlets were morphologically identical with mother plants.
This system can be used for rapid mass clonal propagation of Oxalis triangularis A.st.-Hil, for commercial production, conservation strategies, and producing phytomedicines.
Authors
S. Rittirat, S. Klaocheed, K. Thammasiri
Keywords
6-benzyl-aminopurine, dichlorophenoxy acetic acid, in vitro propagation, multiple shoots, plant growth regulator
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