Articles
Germplasm collection of Vaccinium species in vitro
Article number
1357_50
Pages
353 – 360
Language
English
Abstract
Many epidemiological studies confirm that high consumption of fruits is correlated with a lower incidence of several chronic diseases, mainly for the protective role against cardiovascular, degenerative and proliferative diseases.
Therefore, the nutritional quality of fruit today is becoming an attribute as important as the organoleptic-sensorial quality, and breeding and biotechnological approaches are currently used to increase the content of specific bioactive compounds in fruits.
The PBU has established over the past years a vast collection of Vaccinium species from 8 geographic regions (Austria, Azores, Belarus, Iran, Italy, Madeira, South America, Norway, Slovakia) in vitro.
These include: V. angustifolium, V. arctostaphylos, V. corymbosum, V. cylindracaeum, V. floribundum, V. myrtillus, V. padifolium and V. vitis-ideae. Donor material was collected from different locations worldwide and consisted of seeds, cuttings or entire plants that were used for tissue culture initiation.
Actively growing shoot cultures were initiated according to protocols previously established in the lab, treating every bud like a mericlone.
Plantlets were propagated on WPM and Andersons medium supplemented with growth regulators and kept at 24°C under 16/8 h photoperiod.
Separated cultures of each mericlone were produced.
This collection serves several purposes in the context of 1) maintenance of biodiversity, 2) clonal propagation of selected plant lines or cultivars, 3) establishment of transformation protocols, 4) phytosanitary improvement, and 5) selection of breeding lines.
Viable plant material for replanting to the open field was obtained.
Given the fact, that these shrubs have some ornamental potential, the most vigorous clones, beautifully changing foliage colouration in autumn, will be used in an ornamental and forest plant breeding programme.
Therefore, the nutritional quality of fruit today is becoming an attribute as important as the organoleptic-sensorial quality, and breeding and biotechnological approaches are currently used to increase the content of specific bioactive compounds in fruits.
The PBU has established over the past years a vast collection of Vaccinium species from 8 geographic regions (Austria, Azores, Belarus, Iran, Italy, Madeira, South America, Norway, Slovakia) in vitro.
These include: V. angustifolium, V. arctostaphylos, V. corymbosum, V. cylindracaeum, V. floribundum, V. myrtillus, V. padifolium and V. vitis-ideae. Donor material was collected from different locations worldwide and consisted of seeds, cuttings or entire plants that were used for tissue culture initiation.
Actively growing shoot cultures were initiated according to protocols previously established in the lab, treating every bud like a mericlone.
Plantlets were propagated on WPM and Andersons medium supplemented with growth regulators and kept at 24°C under 16/8 h photoperiod.
Separated cultures of each mericlone were produced.
This collection serves several purposes in the context of 1) maintenance of biodiversity, 2) clonal propagation of selected plant lines or cultivars, 3) establishment of transformation protocols, 4) phytosanitary improvement, and 5) selection of breeding lines.
Viable plant material for replanting to the open field was obtained.
Given the fact, that these shrubs have some ornamental potential, the most vigorous clones, beautifully changing foliage colouration in autumn, will be used in an ornamental and forest plant breeding programme.
Publication
Authors
E.G. Borroto Fernández, V. Hanzer, F. Lok-Lee, D. Kovarova, A. Mokhber, M. Laimer
Keywords
biodiversity, germplasm storage, phytosanitary improvement, genetic improvement, health-related compounds
Online Articles (56)