Articles
Monitoring of surface changes of fresh green asparagus during storage using laser light backscattering imaging
Article number
1382_23
Pages
179 – 186
Language
English
Abstract
Storage conditions play a significant role in maintaining and preserving the quality of fresh horticultural produce.
The evaluation of storage parameters affecting quality and safety is an active research topic.
The aim of this study was to monitor the surface changes of asparagus under different storage conditions using Laser Light Backscattering Imaging (LLBI), microscopic, and digital imaging techniques.
The samples were stored at 2, 10, and 15°C for 12 days.
Stomata size and density, surface color, and backscattering signal parameters such as diameter at 75% (D75), 50% (D50), and 25% (D25) intensity levels were measured.
The results showed that the size and density of stomata on the surface of asparagus spears varied with location.
The saturation peaks in hue spectra increased with storage temperature and time, while the hue angle and light penetration depth decreased.
Furthermore, the hue spectra at 40° demonstrated strong positive correlations with the stomata length (r=0.78) and width (r=0.74), respectively.
Additionally, the laser backscattering width at 75, 50, and 25% intensity levels showed strong negative correlations with stomata width at correlation coefficients of -0.87, -0.85, and -0.87, respectively.
The linear discriminant analysis (LDA) was applied based on LLBI parameters and achieved 97.23% accuracy in cross-validation.
Furthermore, the partial least squares regression model based on laser backscattering imaging parameters predicted the width of the asparagus stomata with high accuracy of R2=0.98, RMSE=0.003 mm, and RPD=8.14. Therefore, LLBI is a promising technique for monitoring the quality and shelf life of asparagus during storage.
The evaluation of storage parameters affecting quality and safety is an active research topic.
The aim of this study was to monitor the surface changes of asparagus under different storage conditions using Laser Light Backscattering Imaging (LLBI), microscopic, and digital imaging techniques.
The samples were stored at 2, 10, and 15°C for 12 days.
Stomata size and density, surface color, and backscattering signal parameters such as diameter at 75% (D75), 50% (D50), and 25% (D25) intensity levels were measured.
The results showed that the size and density of stomata on the surface of asparagus spears varied with location.
The saturation peaks in hue spectra increased with storage temperature and time, while the hue angle and light penetration depth decreased.
Furthermore, the hue spectra at 40° demonstrated strong positive correlations with the stomata length (r=0.78) and width (r=0.74), respectively.
Additionally, the laser backscattering width at 75, 50, and 25% intensity levels showed strong negative correlations with stomata width at correlation coefficients of -0.87, -0.85, and -0.87, respectively.
The linear discriminant analysis (LDA) was applied based on LLBI parameters and achieved 97.23% accuracy in cross-validation.
Furthermore, the partial least squares regression model based on laser backscattering imaging parameters predicted the width of the asparagus stomata with high accuracy of R2=0.98, RMSE=0.003 mm, and RPD=8.14. Therefore, LLBI is a promising technique for monitoring the quality and shelf life of asparagus during storage.
Authors
Z.H. Siyum, T.P. Thanh, M. Höhn, L. Baranyai, L.P.L. Nguyen
Keywords
diffuse reflectance, machine vision, quality assessment, shelf life, microscopy
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