Articles
Organic amendments evaluation based on generation of biocidal compounds after soil biodisinfestation in a humid temperate climate
Article number
1410_10
Pages
63 – 72
Language
English
Abstract
Several mechanisms occur during soil biodisinfestation (BD) with organic amendments (OA) for the control of soil-borne diseases in intensive greenhouse horticultural crops.
In areas and/or seasons with limited solar radiation, it is interesting to reinforce the anaerobic component of those mechanisms.
In anaerobic soil disinfestation (ASD), volatile fatty acids (VFAs) are produced and can exert a biocidal effect against soil-borne pathogens.
This study was carried out in a commercial lettuce greenhouse of the Basque Atlantic area (Northern Spain) that was affected by Meloidogyne incognita. The soil content of various VFAs (formic, acetic, lactic), ethanol, and sugars (glucose, maltose, xylose) were measured at two soil depths (0-15 and 15-30 cm) at the end of BD treatment with four OA mixtures (M1, M2, M3, M4) of various agri-food by-products (fresh cattle manure, sunflower seed husks, wheat bran, beer bagasse and rapeseed cake). A control (non-amended and non-plastic-mulched soil) treatment was also included.
BD treatment was applied for 6 weeks starting on mid-July-2019 with a 30-micron thick transparent total impermeable film (TIF) of polyethylene plastic.
For each treatment and depth, soil samples were taken, and the liquid phase obtained after extraction was analyzed by High Performance Liquid Chromatography (HPLC) with a chromatograph with a refractive index detector.
Significant differences were observed in the content of VFAs, ethanol, and sugars among BD treatments and soil depths.
The M1, M2, M3, and M4 biodisinfestation treatments showed an increase of 16, 50, 25, and 5% in the total organic compound soil content (sum of sugars plus VFAs plus ethanol when compared to the non-treated control, respectively.
The deepest soil layer) showed a significant 15% decrease in the total organic compound soil content when compared to the shallowest layer.
These results show the potential of the anaerobic component of BD treatments in areas and/or seasons with limited solar radiation.
In areas and/or seasons with limited solar radiation, it is interesting to reinforce the anaerobic component of those mechanisms.
In anaerobic soil disinfestation (ASD), volatile fatty acids (VFAs) are produced and can exert a biocidal effect against soil-borne pathogens.
This study was carried out in a commercial lettuce greenhouse of the Basque Atlantic area (Northern Spain) that was affected by Meloidogyne incognita. The soil content of various VFAs (formic, acetic, lactic), ethanol, and sugars (glucose, maltose, xylose) were measured at two soil depths (0-15 and 15-30 cm) at the end of BD treatment with four OA mixtures (M1, M2, M3, M4) of various agri-food by-products (fresh cattle manure, sunflower seed husks, wheat bran, beer bagasse and rapeseed cake). A control (non-amended and non-plastic-mulched soil) treatment was also included.
BD treatment was applied for 6 weeks starting on mid-July-2019 with a 30-micron thick transparent total impermeable film (TIF) of polyethylene plastic.
For each treatment and depth, soil samples were taken, and the liquid phase obtained after extraction was analyzed by High Performance Liquid Chromatography (HPLC) with a chromatograph with a refractive index detector.
Significant differences were observed in the content of VFAs, ethanol, and sugars among BD treatments and soil depths.
The M1, M2, M3, and M4 biodisinfestation treatments showed an increase of 16, 50, 25, and 5% in the total organic compound soil content (sum of sugars plus VFAs plus ethanol when compared to the non-treated control, respectively.
The deepest soil layer) showed a significant 15% decrease in the total organic compound soil content when compared to the shallowest layer.
These results show the potential of the anaerobic component of BD treatments in areas and/or seasons with limited solar radiation.
Authors
S. Larregla, M. Gandariasbeitia, J.A. López-Pérez, M.M. Guerrero, E. Corro, J. Labidi
Keywords
biofumigation, total impermeable film (TIF), biosolarization, anaerobic soil disinfestation (ASD), volatile fatty acids (VFAs), Lactuca sativa, Meloidogyne incognita
Online Articles (22)