Articles
Fundamentals of plant cryopreservation: dormant bud two-step cooling and shoot tip vitrification
Article number
1421_16
Pages
117 – 124
Language
English
Abstract
Plant shoot tip and dormant bud cryopreservation have become routine for many genebank and conservation programs concerned with the long-term storage of non-seed materials.
This is due in part to the development of methods that are applicable across a wide range of diversity within genera as well as a highly skilled workforce that is able to implement difficult procedures.
Successful dormant bud cryopreservation depends on having plant materials at an appropriate physiological state to tolerate subsequent dehydration and cryoexposure.
Effective shoot tip cryopreservation often relies on a combination of treatments that allow tender shoot tips to survive and recover following cryopreservation.
Remarkably, the underlying physiological, biophysical, and molecular mechanisms of successful cryopreservation procedures are not well understood.
Interdisciplinary efforts are revealing new information about how cells interact with cryoprotectant solutions, which allow shoot tips to survive and recover from liquid nitrogen exposure.
This is due in part to the development of methods that are applicable across a wide range of diversity within genera as well as a highly skilled workforce that is able to implement difficult procedures.
Successful dormant bud cryopreservation depends on having plant materials at an appropriate physiological state to tolerate subsequent dehydration and cryoexposure.
Effective shoot tip cryopreservation often relies on a combination of treatments that allow tender shoot tips to survive and recover following cryopreservation.
Remarkably, the underlying physiological, biophysical, and molecular mechanisms of successful cryopreservation procedures are not well understood.
Interdisciplinary efforts are revealing new information about how cells interact with cryoprotectant solutions, which allow shoot tips to survive and recover from liquid nitrogen exposure.
Authors
G.M. Volk, R.M. Bonnart, A.D. Henk, K.Y. Chen, J.C. Bettoni, Q.-C. Wang, H.D. Kreckel, N.E. Levinger
Keywords
antioxidant, clonal, cryoprotectant, gene expression, liquid nitrogen, meristem, regrowth
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