Articles
Preliminary studies for cryopreservation of zygotic explants of Quercus crassipes
Article number
1421_3
Pages
21 – 26
Language
English
Abstract
Currently, oaks are strongly affected by direct felling, changes in land use, deforestation, climate change, pests, and diseases.
Seed banks are an efficient strategy for ex situ conservation of plant species.
Nonetheless, seed banking is not suitable for some species from temperate forest like oaks due to their recalcitrant seeds.
Thus it becomes essential to use techniques such as cryopreservation for the long-term germplasm storage.
The objectives of this study were to identify seed desiccation tolerance and to evaluate the regeneration of zygotic explants (ZE) subjected to different drying times using a flash drying device before being ultra-rapidly immersed in liquid nitrogen (LN). Seed desiccation tolerance was assessed by seed coat ratio (SCR) test, desiccation susceptibility of ZE was evaluated by electrolyte leakage after each desiccation duration, and the regeneration of dried and cryopreserved explants was determined by the in vitro germination after 7 days of culture.
The value of SCR (0.36) showed a P of 0.48. The initial water content (WC) of ZE was 1.01 g H2O g‑1 dry weight.
Although the water loss of the ZE was significant during drying, and the viability was higher than 80% after all desiccation times.
After cryopreservation, the highest regeneration (66.7%) was obtained at 0.15 H2O g‑1 dry weight, at 90 min of desiccation.
Electrolyte leakage was affected by WC, but at 90 min of desiccation there is a balance between electrolyte leakage and WC in function of desiccation duration.
According to these results, seeds of Q. crassipes are more likely to be desiccation tolerant; moreover, the ZE showed tolerance to both desiccation and cryopreservation, suggesting to be suitable candidates for the long-term conservation of Q. crassipes germplasm.
Seed banks are an efficient strategy for ex situ conservation of plant species.
Nonetheless, seed banking is not suitable for some species from temperate forest like oaks due to their recalcitrant seeds.
Thus it becomes essential to use techniques such as cryopreservation for the long-term germplasm storage.
The objectives of this study were to identify seed desiccation tolerance and to evaluate the regeneration of zygotic explants (ZE) subjected to different drying times using a flash drying device before being ultra-rapidly immersed in liquid nitrogen (LN). Seed desiccation tolerance was assessed by seed coat ratio (SCR) test, desiccation susceptibility of ZE was evaluated by electrolyte leakage after each desiccation duration, and the regeneration of dried and cryopreserved explants was determined by the in vitro germination after 7 days of culture.
The value of SCR (0.36) showed a P of 0.48. The initial water content (WC) of ZE was 1.01 g H2O g‑1 dry weight.
Although the water loss of the ZE was significant during drying, and the viability was higher than 80% after all desiccation times.
After cryopreservation, the highest regeneration (66.7%) was obtained at 0.15 H2O g‑1 dry weight, at 90 min of desiccation.
Electrolyte leakage was affected by WC, but at 90 min of desiccation there is a balance between electrolyte leakage and WC in function of desiccation duration.
According to these results, seeds of Q. crassipes are more likely to be desiccation tolerant; moreover, the ZE showed tolerance to both desiccation and cryopreservation, suggesting to be suitable candidates for the long-term conservation of Q. crassipes germplasm.
Authors
S. Campos-Vázquez, M.T. González-Arnao, C.A. Ordoñez-Salanueva
Keywords
flash drying, desiccation duration, liquid nitrogen, electrolyte leakage
Online Articles (17)