Articles
DETECTION AND IDENTIFICATION OF VIROIDS AND VIRAL NUCLEIC ACIDS BY “DOT-BLOT” HYBRIDIZATION
Article number
164_12
Pages
109 – 118
Language
Abstract
A rapid, simple and improved extraction procedure for the detection and identification of viroids and plant viruses by dot-blot hybridization is described.
The entire tissue extraction can be carried out in a single tube and can be used to detect viroids or viruses in a number of plant tissues; i.e., leaves, stems, tubers, shoots, roots and seeds.
The extracts are blotted onto a nitro-cellulose membrane and incubated with 32p-labeled complementary DNA (cDNA) probes to detect and identify the infectious agent.
The sensitivity of detection is comparable to that achieved with phenol-extracted nucleic acids.
The entire tissue extraction can be carried out in a single tube and can be used to detect viroids or viruses in a number of plant tissues; i.e., leaves, stems, tubers, shoots, roots and seeds.
The extracts are blotted onto a nitro-cellulose membrane and incubated with 32p-labeled complementary DNA (cDNA) probes to detect and identify the infectious agent.
The sensitivity of detection is comparable to that achieved with phenol-extracted nucleic acids.
Authors
P. Palukaitis, S. Cotts, M. Zaitlin
Keywords
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