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SOLUBILITY OF CHRYSANTHEMUM CHLOROTIC MOTTLE VIROID IN LICL SOLUTIONS

Article number
164_37
Pages
333 – 340
Language
Abstract
The 2M LiCl insoluble RNA extracts of both the severe and latent strains of chrysanthemum chlorotic mottle viroid (ChCMV-s and CHCMV-l) have higher infectivity titers than do the soluble fractions.
Chrysanthemum stunt viroid, potato spindle tuber viroid, citrus exocortis viroid, and columnea viroid are primarily soluble in 2M LiCl.

ChCMV-s and ChCMV-l migrate at the same rate as the 7s RNA in 5% polyacrylamide gels.
No RNA band unique to ChCMV-infected tissue was observed in 5, 10, or 15% non-denaturing gels.
Attempts to recover infectious ChCMV RNA from gels under fully denaturing conditions were unsuccessful.

Publication
VI International Symposium on Virus Diseases of Ornamental Plants
Authors
S.O. Kawamoto, R.K. Horst, S.-M. Wong
Keywords
Full text
https://doi.org/10.17660/ActaHortic.1985.164.37
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