Articles
INVESTIGATIONS ON PLUM POX (SHARKA) VIRUS IN SPAIN
Article number
193_27
Pages
155 – 160
Language
Abstract
In June 1984, plum pox (sharka) virus (PPV) was detected for the first time in Spain.
Initially the virus was detected by the enzyme-linked immunosorbent assay (ELISA) in Japanese plum trees, cv.
Red Beaut, showing typical symptoms of the disease.
The detection was later confirmed by graft-transmission on GF-305 peach seedlings and also by immunoelectron microscopy.
At present our work aims at establishing the most suitable conditions for detecting PPV by ELISA, obtaining monoclonal antibodies from semi-purified solutions of PPV and determining the real incidence of the virus in Spain.
The state of our work is discussed in the present communication.
Initially the virus was detected by the enzyme-linked immunosorbent assay (ELISA) in Japanese plum trees, cv.
Red Beaut, showing typical symptoms of the disease.
The detection was later confirmed by graft-transmission on GF-305 peach seedlings and also by immunoelectron microscopy.
At present our work aims at establishing the most suitable conditions for detecting PPV by ELISA, obtaining monoclonal antibodies from semi-purified solutions of PPV and determining the real incidence of the virus in Spain.
The state of our work is discussed in the present communication.
Authors
G. Llacer, M. Cambra, A. Lavina, J. Aramburu
Keywords
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