Articles
ISOLATION OF DSRNA FROM PLUM POX VIRUS-INFECTED PLANT TISSUE
Article number
193_29
Pages
167 – 172
Language
Abstract
A method for the isolation of dsRNA from plum pox virus (PPV)-infected plant tissue is described.
Two PPV strains cultivated in Nicotiana clevelandii Gray were detected by immune specific electron microscopy (ISEM), enzyme-linked immunosorbent assay (ELISA) and by isolation of double stranded RNA (dsRNA). All three methods showed similar sensitivity when samples were analysed 7 or 14 days after inoculation.
In samples harvested 21 days after inoculation it was difficult by ISEM or ELISA to detect one strain which had low particle and antigen concentration, while dsRNA was still detected in considerable amounts.
The dsRNA method may help in the detection of PPV in woody hosts in the winter season when no symptom-bearing leaves are available.
Two PPV strains cultivated in Nicotiana clevelandii Gray were detected by immune specific electron microscopy (ISEM), enzyme-linked immunosorbent assay (ELISA) and by isolation of double stranded RNA (dsRNA). All three methods showed similar sensitivity when samples were analysed 7 or 14 days after inoculation.
In samples harvested 21 days after inoculation it was difficult by ISEM or ELISA to detect one strain which had low particle and antigen concentration, while dsRNA was still detected in considerable amounts.
The dsRNA method may help in the detection of PPV in woody hosts in the winter season when no symptom-bearing leaves are available.
Authors
E. Breyel, E. Maiss, R. Casper, F. El-Ouaghlidi
Keywords
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