ELIMINATION OF CONTAMINATING MICRO-ORGANISMS FROM MERISTEM CULTURE OF APPLE ROOTSTOCK M26

In meristem culture of apple rootstock M26, four contaminating organisms were isolated and cultured, i.e. two gram-negative bacteria and two yeasts.

The bacteria were eliminated by applying the cephalosporins cefotaxime and cephalosporin (50 μg/ml) to the culture medium.

Several substances were tested for their toxicity to yeast without harming the plant material, but none was found useful.

Bacterial and Bacteria-like Contaminants of Plant Tissue Cultures

G.B. Poulsen

https://doi.org/10.17660/ActaHortic.1988.225.22