Articles
THREE YEARS FIELD PERFORMANCE OF IN VITRO PROPAGATED JAPANESE WHITE BIRCH(BETULA PLATYPHYLLA VAR. JAPONICA)
Article number
319_22
Pages
167 – 170
Language
Abstract
Tissue culture was adopted to propagate good quality trees of Betula platyphylla Sukatchev var. japonica Hara.
About 100 shoot primordia were obtained from a petiole.
These primordia were elongated and subcultured in the modified MS medium to which indole butyric acid was added.
The clone plantlets thus obtained were habituated in mist house and transplanted in the field at a high density of 40,000 per hectare.
In vitro propagated good-quality clonal plantlets were 1.3 times greater in height after two growth periods and 1.5 times after three as compared with the control seedlings.
Average height and volume after two growing period were 277cm and 1900 cm3 respectively.
It was found that it would be very valuable economically to propagate the high-quality clones of this species by tissue culture technique.
About 100 shoot primordia were obtained from a petiole.
These primordia were elongated and subcultured in the modified MS medium to which indole butyric acid was added.
The clone plantlets thus obtained were habituated in mist house and transplanted in the field at a high density of 40,000 per hectare.
In vitro propagated good-quality clonal plantlets were 1.3 times greater in height after two growth periods and 1.5 times after three as compared with the control seedlings.
Average height and volume after two growing period were 277cm and 1900 cm3 respectively.
It was found that it would be very valuable economically to propagate the high-quality clones of this species by tissue culture technique.
Authors
K. Ishii, T. Kanazashi
Keywords
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