Articles
IMPROVED RATE OF CALLUS AND PLANTLET FROM ANTHER CULTURE OF ASPARAGUS (ASPARAGUS OFFICINALIS L.)
The highest frequency of callus induction was 78.05%.
Callus formation ability differed among individual plants.
The organogenesis were induced when calli were subcultured to MS medium with 0.05–0.2 mg/L BA and 0.05–0.3 mg/L NAA. The highest frequency of stem and pseudo-leaf induction (76.96%) was obtained on the MS medium with 0.1 mg/L NAA and 0.1 mg/L BA, a few callus developed shoots and roots as well.
Clustered shoots were cultured on the half strength MS (Except FeNa2-EDTA) medium with 0.1 mg/L NAA or 2 mg/L IBA and 1–2 mg/L IAA or 2 mg/L IBA and 0.1 mg 1 NAA or 1.5 mg/L IBA and 0.1 mg/L KT, the roots were initiated.
The highest rooting percentage can be raised to 62.37% on ½ MS (Except FeNa-EDTA) with 0.1 mg/L NAA, the roots were normal.
Hardened the regenerative plants in the culture flask with cotton plug in greenhouse with strong light for 15–20 days.
The plantlets had normal pseudo-leaves, strong roots and strong resistance.
The transplanting survival rate can be raised to 84.55%.