Articles
GENETIC MANIPULATION OF PHYTOCHROMES IN TOMATO (Lycopersicon esculentum Mill.): A NOVEL APPROACH TO CROP IMPROVEMENT
Article number
487_9
Pages
93 – 98
Language
Abstract
We are testing the hypothesis that genetic manipulation of phytochrome genes (PHY) can improve agronomic traits in tomato.
Here, we report our analyses of fruit from two different PHY mutants and a line over-expressing a PHYA transgene.
A MoneyMaker line containing a PHYBI mutation has increased yield (total kg) when grown in the greenhouse (74%) or the field (39%). PHYBI mutations do not affect fresh fruit weight (FW), dry weight/fresh weight (DW/FW), total soluble solids (SS), paste viscosity, the number of seeds per fruit, nor fruit color.
A MoneyMaker line containing a PHYA mutation produces fruit with qualities important to the paste industry.
Compared to wild-type (WT) fruit, PHYA mutant fruit have increased DW/FW, more SS, increased paste viscosity, and fewer seeds per fruit.
A transgenic UC-82B line over-expressing PHYA also produces fruit with altered qualities.
Compared to WT fruit, this PHYA over-expressor produces fruit that yield less viscous paste and have more seeds.
Collectively, these results indicate significant potential for improving tomato crops via the genetic manipulation of phytochrome loci.
Here, we report our analyses of fruit from two different PHY mutants and a line over-expressing a PHYA transgene.
A MoneyMaker line containing a PHYBI mutation has increased yield (total kg) when grown in the greenhouse (74%) or the field (39%). PHYBI mutations do not affect fresh fruit weight (FW), dry weight/fresh weight (DW/FW), total soluble solids (SS), paste viscosity, the number of seeds per fruit, nor fruit color.
A MoneyMaker line containing a PHYA mutation produces fruit with qualities important to the paste industry.
Compared to wild-type (WT) fruit, PHYA mutant fruit have increased DW/FW, more SS, increased paste viscosity, and fewer seeds per fruit.
A transgenic UC-82B line over-expressing PHYA also produces fruit with altered qualities.
Compared to WT fruit, this PHYA over-expressor produces fruit that yield less viscous paste and have more seeds.
Collectively, these results indicate significant potential for improving tomato crops via the genetic manipulation of phytochrome loci.
Publication
Authors
R. Alba, M.-M. Cordonnier-Pratt, L.H. Pratt, C.J. Valenzano, S.J. Kays
Keywords
Lycopersicon esculentum, phytochrome, photoreceptors, yield, paste qualities
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