Articles
EFFECTS OF POLY (2-HYDROXYPROPYLDIMETHYAMMONIUM CHLORIDE) TREATMENT ON THE POSTHARVEST LONGEVITY OF CUT ROSES IN RELATION TO STOMATAL BEHAVIOR.
Article number
518_4
Pages
37 – 44
Language
Abstract
Pretreatment with poly (2-hydroxypropyldimethylammonium chloride) (PHPAC), provides well-balanced water relations in cut roses, preventing ‘bent neck’ and extending the vase life.
The influence of PHPAC on stomatal behavior was investigated.
Cut ‘Rote Rose’ roses were treated with 250 ppm PHPAC for 12 hr, then placed in deionized water, and held at 20°C under 12 hr light at 20 μmol/m2/s.
Stomatal conductance of the uppermost five-leaflet leaves was measured in the middle of the light period by fitting the sensor of LI-1600 super porometer (Li-cor Inc., Neb., U.S.A.). Stomatal conductance of leaves treated with PHPAC was lower than that of the Control on day 1, but was higher after day 2 until the end of the experiment.
To evaluate the influence of PHPAC under higher transpiration conditions, detached leaves were placed under the light at 300 μmol/m2/s.
The stomatal conductance decreased during the first 3 days, but increased thereafter as compared with that in the control.
The influence of PHPAC on stomatal behavior was investigated.
Cut ‘Rote Rose’ roses were treated with 250 ppm PHPAC for 12 hr, then placed in deionized water, and held at 20°C under 12 hr light at 20 μmol/m2/s.
Stomatal conductance of the uppermost five-leaflet leaves was measured in the middle of the light period by fitting the sensor of LI-1600 super porometer (Li-cor Inc., Neb., U.S.A.). Stomatal conductance of leaves treated with PHPAC was lower than that of the Control on day 1, but was higher after day 2 until the end of the experiment.
To evaluate the influence of PHPAC under higher transpiration conditions, detached leaves were placed under the light at 300 μmol/m2/s.
The stomatal conductance decreased during the first 3 days, but increased thereafter as compared with that in the control.
The effect of PHPAC on stomatal closure was examined by immersing the fragments of Commelina communis L. leaves in PHPAC or ABA solutions at various concentrations for 3 hr under the light at 435 μmol/m2/s.
The stomatal aperture was observed microscopically by measuring ‘width/maximum length’ using the digital image processing method on Mitsubishi Kagaku image processing network system.
PHPAC showed an ABA-like effect, i.e., stomatal closure, although the effect greatly depended on the concentration treated.
PHPAC was suggested to control the transpiration rate by closing stomata propriately and thus prolong the vase life of cut roses.
Authors
M. Hanafusa, M. Doi
Keywords
cut rose, preservative, poly (2-hydoroxypropyldimethyammonium chloride), stomatal conductance, stomatal closure
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