Articles
MUTATION INDUCTION BY ION BEAM IN ORNAMENTAL PLANTS – EFFECTS ON SEED GERMINATION AND DNA POLYMORPHISM IN EUSTOMA GRANDIFLORUM
Article number
522_12
Pages
123 – 126
Language
Abstract
Seeds of Eustoma grandiflorum were irradiated under aseptic conditions with a 50-MeV 4He2+ beam 3 and 10 days after imbibition.
The seeds that were irradiated 3 days after imbibition were not affected by the irradiation.
However, less than 5% of the seeds irradiated 10 days after imbibition survived after 4 weeks in culture.
At the time of irradiation, these seeds already had elongated radicles.
Total DNA was analyzed using 10-mer random primers.
DNA polymorphism was detected in the plants from ion beam-irradiated seeds.
The seeds that were irradiated 3 days after imbibition were not affected by the irradiation.
However, less than 5% of the seeds irradiated 10 days after imbibition survived after 4 weeks in culture.
At the time of irradiation, these seeds already had elongated radicles.
Total DNA was analyzed using 10-mer random primers.
DNA polymorphism was detected in the plants from ion beam-irradiated seeds.
Authors
S. Ohki, Y. Sakai, K. Chatani, K. Koreeda
Keywords
Eustoma grandiflorum, DNA polymorphism, ion beam, mutation breeding, RAPD
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