Articles
COMPARISON OF HEAT, SONICATION AND ULTRAVIOLET IRRADIATION IN ELIMINATING PYTHIUM APHANIDERMATUM ZOOSPORES IN RECIRCULATING NUTRIENT SOLUTION
Article number
532_16
Pages
137 – 144
Language
Abstract
Recirculating (‘closed’) hydroponics optimizes fertilizer and water use and achieves zero discharge of nutrient solution into the environment.
Sterilization of the solution before recirculation is needed to prevent spread of pathogens carried in the solution.
Unlike heat pasteurization and ultraviolet (UV) irradiation methods for sterilization, sonication has not been investigated in detail.
This study compares sonication with heat and UV in the elimination of Pythium zoospores and the effect of sonication on Pseudomonas chlororaphis, a bioagent.
It has been found that all Pythium zoospores succumbed after 1.5 min of sonication an amplitude of 120 um but the majority of Pseudomonas bacteria survived.
Thus sonication apparently provides selective elimination of Pythium zoospores and compares favourably to heat and UV. The advantages and disadvantages of each of these methods will be discussed.
Sterilization of the solution before recirculation is needed to prevent spread of pathogens carried in the solution.
Unlike heat pasteurization and ultraviolet (UV) irradiation methods for sterilization, sonication has not been investigated in detail.
This study compares sonication with heat and UV in the elimination of Pythium zoospores and the effect of sonication on Pseudomonas chlororaphis, a bioagent.
It has been found that all Pythium zoospores succumbed after 1.5 min of sonication an amplitude of 120 um but the majority of Pseudomonas bacteria survived.
Thus sonication apparently provides selective elimination of Pythium zoospores and compares favourably to heat and UV. The advantages and disadvantages of each of these methods will be discussed.
Authors
J.C. Tu, W.-Z. Zhang
Keywords
Heat, sonication, ultraviolet irradiation, Pythium
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