Articles
VARIETAL VARIATION IN MICROSATELLITE AND CAPS FOR ETHYLENE-RELATED GENES AND ITS POSSIBLE ASSOCIATION WITH AGRONOMIC CHARACTERS IN MELON (CUCUMIS MELO)
Article number
588_50
Pages
313 – 316
Language
English
Abstract
Shelf-life of fruit is one of the most important traits in melon breeding.
Shelf-life of climacteric fruits is closely related with ethylene whose biosynthesis is controlled by ACC synthase and ACC oxidase genes.
As the first step to develop selection markers for shelf-life in melon (Cucumis melo), microsatellite and cleaved amplified polymorphic sequence (CAPS) polymorphisms were analyzed in two ACC synthase genes (CMe-ACS1, CMe-ACS2) and two ACC oxidase genes (CMe-ACO1, CMe-ACO3), resulting in the establishment of six microsatellite and two CAPS markers.
Five markers were developed in two genes, CMe-ACS1 and CMe-ACO1, which were expressed during fruit ripening.
A highly polymorphic marker was found in CMe-ACS2, where the number of simple sequence repeat of (TA) was highly variable among cultivars, ranging from seven to thirty-seven.
Insertion/deletion of (T)n and (A)n was also detected in CMe-ACS2. CAPS and microsatellite markers specific to vars. makuwa and conomon, local varieties in East Asia, were detected in four markers, Acs1-c1, Acs2-ms1, Aco1-ms2 and Aco3-ms1, and could be useful markers for phylogenetic analysis in melon.
Shelf-life of climacteric fruits is closely related with ethylene whose biosynthesis is controlled by ACC synthase and ACC oxidase genes.
As the first step to develop selection markers for shelf-life in melon (Cucumis melo), microsatellite and cleaved amplified polymorphic sequence (CAPS) polymorphisms were analyzed in two ACC synthase genes (CMe-ACS1, CMe-ACS2) and two ACC oxidase genes (CMe-ACO1, CMe-ACO3), resulting in the establishment of six microsatellite and two CAPS markers.
Five markers were developed in two genes, CMe-ACS1 and CMe-ACO1, which were expressed during fruit ripening.
A highly polymorphic marker was found in CMe-ACS2, where the number of simple sequence repeat of (TA) was highly variable among cultivars, ranging from seven to thirty-seven.
Insertion/deletion of (T)n and (A)n was also detected in CMe-ACS2. CAPS and microsatellite markers specific to vars. makuwa and conomon, local varieties in East Asia, were detected in four markers, Acs1-c1, Acs2-ms1, Aco1-ms2 and Aco3-ms1, and could be useful markers for phylogenetic analysis in melon.
Publication
Authors
Y. Akashi, H. Ezura, Y. Kubo, M. Masuda, K. Kato
Keywords
phylogeny
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