Articles
CLONING AND CHARACTERIZATION OF A MELON E8-LIKE GENE
Article number
588_59
Pages
363 – 367
Language
English
Abstract
A genomic DNA encoding an E8-like gene (CmE8, accession number AB071820) was isolated from melon and its nucleotide sequence was determined.
The CmE8 consisted of three exons and two introns.
The number and size of the exons and the location of introns were identical to those in tomato E8 gene.
The deduced amino acid sequence of CmE8 consisted of 367 amino acids, and it was similar to those of 2-oxoglutarate-dependent dioxygenase (2-ODD) homologues from apricot, tomato (E8 and E8-like genes) and Chataransus roseu. RT-PCR analysis indicated that mRNA levels of CmE8 increased in mesocarp of ripening melon fruit.
Nucleotide sequences resembling ethylene-response elements were found in the 5- flanking region of CmE8 gene.
The CmE8 consisted of three exons and two introns.
The number and size of the exons and the location of introns were identical to those in tomato E8 gene.
The deduced amino acid sequence of CmE8 consisted of 367 amino acids, and it was similar to those of 2-oxoglutarate-dependent dioxygenase (2-ODD) homologues from apricot, tomato (E8 and E8-like genes) and Chataransus roseu. RT-PCR analysis indicated that mRNA levels of CmE8 increased in mesocarp of ripening melon fruit.
Nucleotide sequences resembling ethylene-response elements were found in the 5- flanking region of CmE8 gene.
Publication
Authors
A. Fujimori, J.A.T. Pariasca, M. Lida, S. Uchiyama, T. Ozawa, T. Hirabayashi, H. Nakagawa, T. Sato
Keywords
Cucumis melo, 2-oxoglutarate-dependent dioxygenase, fruit ripening, ethylene
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