Articles
DETECTION OF ERWINIA AMYLOVORA IN AND ON APPLE TISSUE USING PCR
Article number
590_23
Pages
163 – 166
Language
English
Abstract
A PCR-based method for detection of epiphytic and endophytic Erwinia amylovora with the application of minicolumns (Genomic DNA Prep Plus) for DNA purification and primers complementary to 23S rDNA (Maes et al., 1996) was developed.
All 18 Polish strains of E. amylovora originating from various host plants and geographical regions produced specific PCR fragments but no product was obtained with 5 strains of bacteria belonging to other genera.
The method allows detection of as few as 300-400 cfu/ml of leaf washings or shoot sample.
Various contaminants occurring on and in apple tissue, including pesticide residues, did not interfere with the assay.
All 18 Polish strains of E. amylovora originating from various host plants and geographical regions produced specific PCR fragments but no product was obtained with 5 strains of bacteria belonging to other genera.
The method allows detection of as few as 300-400 cfu/ml of leaf washings or shoot sample.
Various contaminants occurring on and in apple tissue, including pesticide residues, did not interfere with the assay.
Publication
Authors
J. Pulawska, P. Sobiczewski
Keywords
Erwinia amylovora, fire blight, detection, PCR inhibitors, pesticides
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