Home > XXVI International Horticultural Congress: Elegant Science in Floriculture > PLOIDY IDENTIFICATION IN M1 AND M2 GENERATION OF VIOLA x WITTROCKIANA GAMS. AFTER MUTAGENESIS

Articles

PLOIDY IDENTIFICATION IN M₁ AND M₂ GENERATION OF VIOLA x WITTROCKIANA GAMS. AFTER MUTAGENESIS

Article number

624_69

Pages

501 – 509

Language

English

Abstract

The aim of this study was to develop a novel approach to produce autopolyploid plants of Viola x wittrockiana Gams. Pirna group.
Two variants of colchicine treatment were used to induce chromosome doubling and their efficiency was compared.
Early detection of novel ploidy levels was performed by screening guard cell size, leaf index value (leaf blade length/width) and other morphological characteristics.
Subsequent screening of novel ploidy levels was performed by DNA flow cytometry (FCM).

Publication

XXVI International Horticultural Congress: Elegant Science in Floriculture

Authors

I. Ajalin, F. Kobza, J. Doležel

Keywords

Flow cytometry, polyploidy, ploidy identification, chemomutagenesis, colchicine, leaf index, guard cell size

Full text

https://doi.org/10.17660/ActaHortic.2003.624.69

Online Articles (76)

2. ETHICAL GERMPLASM ACQUISITION AND DEVELOPMENT OF NEW FLORICULTURAL CROPS

B.E. Corr

3. NEW ORNAMENTAL CROP INTRODUCTION: A MODEL OF COOPERATION BETWEEN INDUSTRY AND ACADEMIA

A.M. Armitage

4. THE HERBACEOUS ORNAMENTAL PLANT GENEBANK: ITS ROLE IN THE FLORICULTURE INDUSTRY

D. Tay

5. BREEDING AND DEVELOPMENT OF NEW ORNAMENTAL PLANTS FROM NORTH AMERICAN NATIVE TAXA

J. Ault

6. INBREEDING DEPRESSION IN SEED-PROPAGATED LILIUM X-FORMOLONGI INBREDS

N.O. Anderson | B. Dunn

7. MODE OF INHERITANCE AND ALLELE EXPRESSION OF THREE MAJOR ANTHOCYANADINS IN EUSTOMA GRANDIFLORUM CULTIVARS

A.F.M.J. Uddin | F. Hashimoto | Y. Sakata

8. RESULTS AND NEW TRENDS IN BREEDING OF ROMANIAN GLADIOLUS

M. Cantor | D. Zaharia | I. Pop

9. GLADIOLUS SCABRIDUS – THE ROAD TO CONSERVATION AND COMMERCIALIZATION

T.B. Campbell | J.P. Bower

10. BREEDING OF DWARF TUBEROSE (POLIANTHES TUBEROSA L.)

T.-M. Shen | K.-L. Huang | R.-S. Shen | B.-S. Du

11. INTERSPECIFIC AND INTERGENERIC HYBRIDIZATION IN BROMELIACEAE

I. Vervaeke | R. Deroose | M.P. De Proft

12. DEVELOPMENT AND EVALUATION OF CUPHEA PULCHRA AND CUPHEA SCHUMANNII AS NEW LANDSCAPE ORNAMENTALS

J.H. Leopold | J.D. Metzger | S.A. Graham

13. TRIGGERPLANTS (STYLIDIUM; STYLIDIACEAE): A NEW FLORAL AND HORTICULTURAL CROP WITH PRELIMINARY ANALYSIS OF HARDINESS

D.W. Darnowski

14. EFFECTS OF RHIZOME STORAGE TIME AND TEMPERATURE ON GROWTH AND CARBOHYDRATE CONTENT OF ORNAMENTAL GINGER

M. del Pilar Paz | J.S. Kuehny | G. McClure | R. Criley

15. MORPHOLOGY, GROWTH AND FLOWERING BEHAVIOR OF CURCUMA ZEDOARIA

N. Maciel | R.A. Criley

16. INFLUENCE OF INDUCED DORMANCY AND BULB STORAGE TEMPERATURE ON FLOWERING OF VELTHEIMIA BRACTEATA ‘LEMON FLAME’

J.L. Ehlers | P.J. Jansen van Vuuren | L. Morey

17. PEROXIDASE ACTIVITY DURING ROOTING OF CUTTINGS IN EBENUS CRETICA/ A PRELIMINARY STUDY

T.D. Syros | T.A. Yupsanis | A.S. Economou

18. BIODIVERSITY OF ORNAMENTAL PLANTS WITH POTENTIAL AS INDOOR PLANTS IN ROMANIA

19. UNLOCKING THE HORMONAL AND MOLECULAR CONTROLS OF FLOWERING

R.W. King

20. ANTHOCYANIN AND CAROTENOID PIGMENTS IN SPATHE TISSUE FROM SELECTED ZANTEDESCHIA HYBRIDS

D.H. Lewis | H.S. Arathoon | E.E. Swinny | S.C. Huang | K.A. Funnell

21. TEMPERATURE AND PHOTOPERIODIC EFFECTS ON GROWTH AND FLOWERING OF ZYGOPETALUM REDVALE ‘FIRE KISS’ ORCHIDS

R.G. Lopez | E.S. Runkle | R.D. Heins | C.M. Whitman

22. NATURAL FLOWER INITIATION IN SPATHIPHYLLUM: INFLUENCE OF DAY LENGTH AND LIGHT INTENSITY

E. Oyaert | L. Heemers | S. Roels | P.C. Debergh | E. Volckaert

23. INCREASED ANTHOCYANIN ACCUMULATION IN FLOWERS AND FOLIAGE AT ELEVATED TEMPERATURES IS AFFECTED BY MAGNESIUM TREATMENT

M. Oren-shamir | A. Nissim-Levi | R. Ovadia | S. Kagan | L. Shaked-Sachray

24. DIFFERENTIAL TEMPERATURE (DIF) EFFECTS ON THE GROWTH, MORPHOLOGY AND FLOWERING OF ANTIRRHINUM MAJUS L. (SNAPDRAGON) CULTIVARS

H. Shang | G.F. Deitzer | J.D. Lea-Cox

25. EFFECTS OF LOW LIGHT LEVELS DURING THE DARK PERIOD ON FLOWER DEVELOPMENT IN EUPHORBIA PULCHERRIMA

26. TEMPERATURE AFFECTS FLOWER INITIATION AND DEVELOPMENT RATE OF IMPATIENS, PETUNIA, AND VIOLA

N.S. Mattson | J.E. Erwin

27. PARTITIONING OF CARBOHYDRATES IN THE BULB OF ORIENTAL LILY ‘STARGAZER’ IS INFLUENCED BY SUPPLEMENTAL LIGHTING DURING FORCING

J. Treder

28. PRODUCTIVITY AND PERIODICITY OF FLOWERING IN HELICONIA ORTHOTRICHA CULTIVARS

R.A. Criley | J.Y. Uchida | Z.F. Zhengqing Fu

29. EFFICACY OF PLANT GROWTH REGULATORS ON THE GROWTH OF ARGYRANTHEMUM FRUTESCENS ‘COMET PINK’

J.L. Gibson | T.J. Cavins | L. Greer | B.E. Whipker | J.M. Dole

30. EFFECT OF PLANTING DEPTH ON GROWTH AND FLOWERING OF ORNITHOGALUM SAUNDERSIAE BAK

W. Kariuki

31. COLD REQUIREMENTS FOR FLOWERING OF FORGET-ME-NOT

M.G. Karlsson | J.W. Werner

32. PERSISTENCE OF PLANT GROWTH REGULATOR EFFECTS ON PERENNIAL PLANTS IN THE NURSERY

J.G. Latimer | H.L. Scoggins | T.J. Banko

33. INTERACTION OF METHYL JASMONATE AND 1-AMINOCYCLOPROPANE-1-CARBOXYLIC ACID (ACC) IN REGULATION OF SOME PHYSIOLOGICAL PROCESSES IN UNCOOLED AND COOLED TULIP BULBS

34. TEMPERATURE CONTROLS THE RATE OF DEVELOPMENT IN SHORT-DAY AND LONG-DAY PLANTS DURING VEGETATIVE STAGE OF ONTOGENESIS

M.I. Sysoyeva | E.F. Markovskaya | T.G. Kharkina

35. INFLUENCE OF SUPPLEMENTAL LIGHTING ON FRESH AND DRY MATTER ACCUMULATION OF ORIENTAL LILIES DURING FORCING

J. Treder

36. EFFECT OF RINGING AND BA FOLIAGE TREATMENT ON CUT FLOWER QUALITY OF PRUNUS x KEIO-ZAKURA OHWI

N. Yamasaki

37. EFFECT OF DIFFERENT GREENHOUSE ON THE FLOWERING OF ALSTROEMERIA

38. A MECHANISTIC STUDY ON THE EFFECTS OF PHOTOPERIODIC LIGHTING ON FLOWERING TIME AND FLOWER QUALITY OF ‘HUNTING SONG’ GLADIOLUS

J. Feng | Q. Zhang

39. A MECHANISTIC STUDY ON THE EFFECTS OF SOIL-HEATING ON FLOWERING TIME AND FLOWER QUALITY OF ‘HUNTING SONG’ GLADIOLUS

J. Feng | Q. Zhang

40. EFFECT OF END-OF-DAY FAR RED LIGHT ON THE RESPONSES TO FLUCTUATIN DAY/NIGHT TEMPERATURES IN CUCUMBER AND ARABIDOPSIS

R. Moe | J. Xiong | G.G. Patil | S. Torre | E. Thingnaes

41. GROWTH AND FLOWERING OF KALANCHOE ‘RAKO’ AS AFFECTED BY CONCENTRATION OF PACLOBUTRAZOL AND UNICONAZOLE

M.I. Young Lee | N. Hee Choi | B. Ryong Jeong

42. PHOTOSYNTHETIC FEATURES OF THREE GERBERA CULTIVARS IN LOW LIGHT

N. Sevelius

43. IN SEARCH OF ETERNAL YOUTH: THE DELAY OF POSTHARVEST SENESCENCE IN FLOWERS

W.R. Woodson | M.L. Jones

44. GENETIC ANALYSIS OF ADVANCED POPULATIONS IN ANTIRRHINUM MAJUS (SNAPDRAGON)

E.T. Martin | M.E. Stimart

45. CHOOSING A MODEL SYSTEM FOR STUDIES OF LEAF RE-GREENING

E.T. Paparozzi | M.E. Conley

46. LEAF PHOTOSYNTHESIS, FLOWER RESPIRATION AND FLOWER LONGEVITY OF POT ROSES INFLUENCED BY CULTIVAR AND GROWING ENVIRONMENT

J.A.S. Grossi | H.B. Pemberton | J.T. Baker

47. INTERACTIVE EFFECTS OF DURATION OF LONG-DAY PERIOD AND PLANT DENSITY ON EXTERNAL QUALITY OF CUT CHRYSANTHEMUM

S.M.P. Carvalho | V.B. Dhonukshe | E. Heuvelink

48. USE OF MOLECULAR MARKERS TO IMPROVE CUT FLOWERS LONGEVITY IN CARNATION

L. De Benedetti | G. Burchi | S. Bruna | A. Mercuri | T. Schiva

49. PHYTOHORMONES REGULATE SENESCENCE OF CUT CHRYSANTHEMUM

W. Guo | L. Zheng | Z. Zhang | W. Zeng

50. TREATMENT WITH THIDIAZURON FOR PREVENTING LEAF YELLOWING IN CUT TULIPS AND CHRYSANTHEMUM

A. Ferrante | F. Tognoni | A. Mensuali-Sodi | G. Serra

51. EFFECT OF PULSING WITH AgNO₃ OR STS ON THE ABSORPTION AND DISTRIBUTION OF SILVER AN THE VASE LIFE OF CUT ROSE ‘RED SANDRA’

K.C. Son | H.J. Byoun | M.H. Yoo

52. SCHEDULING OF GLADIOLUS, CELOSIA, HELIANTHUS, AND ZINNIA

J.B. Young | J.S. Kuehny | P.C. Branch

53. EFFECT OF STORAGE OF CUTTINGS ON THE ROOTING OF DIFFERENT CULTIVARS OF CUT CHRYSANTHEMUMS (DENDRANTHEMA GRANDIFLORA TZVELEV) FOR TWO DIFFERENT SEASONS

J.B.S.A. de Almeida | K.F.L. Pivetta

54. DEHYDRATION OF ANNUAL CHRYSANTHEMUM (C. CORONARIUM)

D.S. Dahiya | D. Unnikrishnan | A.K. Gupta | S.K. Sehrawat | S. Siddiqui

55. THE POSTHARVEST LIFE OF PULSED GLADIOLUS SPIKES: THE EFFECT OF PRESERVATIVE SOLUTIONS

P.V. Singh | M. Sharma

56. EFFECT OF PULSING, HOLDING AND LOW TEMPERATURE STORAGE ON KEEPING QUALITY OF ASIATIC LILY HYBRIDS

S.S. Sindhu | N.S. Pathania

57. EFFECTS OF NITROGEN AND POTASSIUM FERTILIZATION ON YIELD AND VASE LIFE OF ORANGE JESSAMINE

R.H. Stamps

58. THE INFLUENCE OF TEMPERATURE ON FLOWER OPENING, VASE LIFE AND TRANSPIRATION OF CUT ROSES AND CARNATIONS

T. Teklic | N. Paradzikovic | V. Vukadinovic

59. EFFICACY OF FLURPRIMIDOL (TOPFLOR) ON BEDDING PLANTS

B.E. Whipker | I. McCall | J.L. Gibson | T.J. Cavins

60. THE POTENTIAL USE OF LYSOPHOSPHATIDYLETHANOLAMINE(LPE), A NATURAL LIPID, TO LENGTHEN THE VASE LIFE OF SHORT-LIVED ‘LAVANDE’ AND ‘SENSATION’ ROSES

A. Snider | J.P. Palta | T. Peoples

61. POST HARVEST LIGHT INTENSITY AND TEMPERATURE ON CARBOHYDRATE LEVELS AND VASE LIFE OF CUT FLOWERS

H.A. Pandya | O.P. Saxena

62. GENERATION OF NEW ORNAMENTAL VARIETIES THROUGH GENETIC MODIFICATION OF PIGMENT BIOSYNTHESIS

63. GENETICS AND BIOCHEMISTRY OF FLOWER COLOR IN STOKES ASTER

J. Gaus | D. Werner | L. Gettys | R. Griesbach

64. ROL GENES AND NEW GENOTYPES OF LIMONIUM GMELINII THROUGH AGROBACTERIUM- MEDIATED TRANSFORMATION

A. Mercuri | L. Anfosso | G. Burchi | L. De Benedetti | T. Schiva

65. CHARACTERIZATION OF GENE EXPRESSION IN ROOTS OF CYPRIPEDIUM PARVIFLORUM VAR. PUBESCENS INCUBATED WITH A MYCORRHIZAL FUNGUS

J.I. Watkinson | G.E. Welbaum

66. TRANSFORMATION OF AFRICAN VIOLET (SAINTPAULIA IONANTHA) WITH GLUCANASECHITINASE GENES USING AGROBACTERIUM TUMEFACIENS

M.S. Narendra Ram | S. Mohandas

67. DNA FINGERPRINTING OF CHRYSANTHEMUM CULTIVARS USING RAPDs

S.K. Sehrawat | R. Kumar | D.S. Dahiya | K.S. Boora | R. Yadav

68. IN VITRO MUTAGENESIS STUDIES IN GLADIOLUS FOR INDUCTION OF RESISTANCE TO FUSARIUM OXYSPORUM F.SP. GLADIOLI

N.S. Pathania | R.L. Misra

69. AN INNOVATIVE TECHNIQUE FOR DISEASE RESISTANCE BREEDING: GAMETOPHYTIC APPROACH

Tejaswini

70. PLOIDY IDENTIFICATION IN M₁ AND M₂ GENERATION OF VIOLA x WITTROCKIANA GAMS. AFTER MUTAGENESIS

I. Ajalin | F. Kobza | J. Doležel

71. PRODUCTION OF VIRUS TESTED GLADIOLI THROUGH IN VITRO AND IN VIVO TECHNIQUES

M. Mangal | S.V. Bhardwaj | A. Handa | K.K. Jindal

72. EFFECT OF MEDIUM COMPONENTS ON IN VITRO TUBERIZATION OF GLORIOSA ROTHSCHILDIANA O’BRIEN

D. Kozak

73. MICROPROPAGATION OF ORNITHOGALUM SAUNDERSIAE BAK.

W. Kariuki | S. Kako

74. MICROPROPAGATION OF DENDROBIUM HYBRIDS THROUGH SHOOT TIP CULTURE

M.S. Kim | J.Y. Kim

75. STUDIES ON MICROPROPAGATION OF TULIPS AND DAFFODILS

Y.D. Sharma | S.B. Kanwar

76. LIGHT EFFECTS ON WAX BEGONIA: PHOTOSYNTHESIS, GROWTH RESPIRATION, AND MAINTENANCE RESPIRATION

M.N. Sainath Krishna | M.W. Van Iersel