Articles
THE OCCURRENCE OF PPV IN CHERRY TREES IN THE CZECH REPUBLIC
Article number
657_35
Pages
237 – 244
Language
English
Abstract
Plum pox virus is the causal agent of sharka disease, one of the most harmful viral diseases of stone fruits.
After the first reports of PPV on sour and sweet cherry in Moldova and Italy in 1994, the virus has been recently reported in cherry in Hungary and Bulgaria (1998) and in Romania (2002). Sharka is currently considered one of the most injurious virus diseases of plum, apricot and peach in Czech Republic.
In order to check the presence and distribution of PPV in cherry, from 1999 a survey in a cherry cultivars collection in Holovousy, Czech Republic, has been carried out. 41 cherry trees, representative of the collection, some of them showing typical sharka symptoms (light green, yellow irregular vein banding and ring-spots), have been selected.
Samples have been analysed by ELISA for PPV, PDV, PNRSV and ACLSV detection, and by RT-PCR using PPV 3’NCR specific primers on total nucleic acids extracted from leaves.
ELISA tests resulted negative for PPV and only sometimes suspected, while 7 of the 41 analysed samples were positive in RT-PCR, and 2 of the respective PCR products were cloned into TA cloning kit (Invitrogen) and sequenced.
Among the other viruses tested, PDV was prevalent, sometimes in mixed infection with PNRSV and/or ACLSV.
After the first reports of PPV on sour and sweet cherry in Moldova and Italy in 1994, the virus has been recently reported in cherry in Hungary and Bulgaria (1998) and in Romania (2002). Sharka is currently considered one of the most injurious virus diseases of plum, apricot and peach in Czech Republic.
In order to check the presence and distribution of PPV in cherry, from 1999 a survey in a cherry cultivars collection in Holovousy, Czech Republic, has been carried out. 41 cherry trees, representative of the collection, some of them showing typical sharka symptoms (light green, yellow irregular vein banding and ring-spots), have been selected.
Samples have been analysed by ELISA for PPV, PDV, PNRSV and ACLSV detection, and by RT-PCR using PPV 3’NCR specific primers on total nucleic acids extracted from leaves.
ELISA tests resulted negative for PPV and only sometimes suspected, while 7 of the 41 analysed samples were positive in RT-PCR, and 2 of the respective PCR products were cloned into TA cloning kit (Invitrogen) and sequenced.
Among the other viruses tested, PDV was prevalent, sometimes in mixed infection with PNRSV and/or ACLSV.
Publication
Authors
M. Navrátil, D. afářová, A. Crescenzi, A. Fanigliulo, S. Comes, K. Petrzik, R. Kare¿ová
Keywords
Plum pox virus, Prunus avium, sweet cherry cultivars, ELISA, RT-PCR
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