Articles
MICROPROPAGATION OF SWEET POTATO UNDER CO2-ENRICHED CONDITION BY USING A NOVEL DISPOSABLE, GAS-PERMEABLE FILM CULTURE VESSEL
Article number
670_18
Pages
157 – 166
Language
English
Abstract
To overcome various disadvantages of conventional culture vessels for micropropagation, a novel disposable vessel has been developed.
The vessel, termed the Vitron, is made of a multi-layered OTP® film (Otsuka Techno Co.
Ltd., Japan) and is supported by a polypropylene frame.
The film possesses superior properties such as: high light transmittance, low water vapor transmittance and thermal stability and in particular, high gas-permeability.
The purpose of this study is to investigate the applicability of the Vitron vessel for the micropropagation of sweet potato Naruto Kintoki by comparing the in vitro and ex vitro growth of plantlets cultured in gas permeable Vitron and in a sealed vessel made of polycarbonate.
Single nodal explants, which were excised from the multiple shoots derived from shoot-tip culture, were cultured in Vitron and polycarbonate vessels on 3% sugar-containing agar Murashige and Skoog (1962) medium (MS) and placed under 3000 ppm CO2-enriched condition at a low photosynthetic photon flux density (PPFD) (45 μmol m-2 s-1). The in vitro, ex vitro growth and the net photosynthetic rate of in vitro plantlets were significantly enhanced in the Vitron when compared to those cultured in a polycarbonate vessel.
The effects of various CO2 concentrations and light intensity on the in vitro and ex vitro growth of plantlets by using this novel Vitron vessel were also examined.
The explants were cultured on the same MS medium under low PPFD at various CO2 concentrations: 1000, 2000, 3000 ppm or control (400 ppm) and they were also cultured under 3000 ppm CO2– enriched condition at various PPFD: 30, 45, 60, 75 and 90 μmol m-2 s-1. The best results for in vitro and ex vitro growth of plantlets were obtained for 3000 ppm CO2 enrichment condition and 75 μmol m-2 s-1 PPFD. The results suggest that the novel Vitron vessel, when placed under the two conditions, could replace the conventional culture vessel for micropropagation of sweet potato Naruto Kintoki.
The vessel, termed the Vitron, is made of a multi-layered OTP® film (Otsuka Techno Co.
Ltd., Japan) and is supported by a polypropylene frame.
The film possesses superior properties such as: high light transmittance, low water vapor transmittance and thermal stability and in particular, high gas-permeability.
The purpose of this study is to investigate the applicability of the Vitron vessel for the micropropagation of sweet potato Naruto Kintoki by comparing the in vitro and ex vitro growth of plantlets cultured in gas permeable Vitron and in a sealed vessel made of polycarbonate.
Single nodal explants, which were excised from the multiple shoots derived from shoot-tip culture, were cultured in Vitron and polycarbonate vessels on 3% sugar-containing agar Murashige and Skoog (1962) medium (MS) and placed under 3000 ppm CO2-enriched condition at a low photosynthetic photon flux density (PPFD) (45 μmol m-2 s-1). The in vitro, ex vitro growth and the net photosynthetic rate of in vitro plantlets were significantly enhanced in the Vitron when compared to those cultured in a polycarbonate vessel.
The effects of various CO2 concentrations and light intensity on the in vitro and ex vitro growth of plantlets by using this novel Vitron vessel were also examined.
The explants were cultured on the same MS medium under low PPFD at various CO2 concentrations: 1000, 2000, 3000 ppm or control (400 ppm) and they were also cultured under 3000 ppm CO2– enriched condition at various PPFD: 30, 45, 60, 75 and 90 μmol m-2 s-1. The best results for in vitro and ex vitro growth of plantlets were obtained for 3000 ppm CO2 enrichment condition and 75 μmol m-2 s-1 PPFD. The results suggest that the novel Vitron vessel, when placed under the two conditions, could replace the conventional culture vessel for micropropagation of sweet potato Naruto Kintoki.
Authors
D. Thi Thanh Giang, M. Tanaka
Keywords
film vessel, photomixotrophic, sweet potato, carbon dioxide, tissue culture
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