Articles
IN VITRO REGENERATION OF PARASERIANTHES FALCATARIA (L.) NIELSEN
Article number
692_22
Pages
167 – 172
Language
English
Abstract
In vitro regeneration of Paraserianthes falcataria (L.) Nielsen was achieved through axillary shoot multiplication.
Single-node segments from six-day-old seedlings were used as explants and cultured on modified MS (Murashige and Skoog, 1962) medium.
The addition of 0.01 – 1.0 µM indole acetic acid (IAA) in combination with 0.1 – 1.0 µM benzyl adenine (BA) induced axillary shoot proliferation.
During the first two-weeks in culture, 4-15 shoots per explant were proliferated.
The highest number of shoots was obtained from single-node explants cultured on medium supplemented with 0.1 µM IAA and 0.5 µM BA. After three-weeks in culture, up to 23 shoots per explant were produced.
Lengthening the period of culture produced no significant increase in shoot proliferation.
In the last week of a 4-week period of culture, the shoot multiplication rate was very low.
Regenerated shoots were elongated on MS medium without growth regulators.
Shoots were rooted on soil during the period of acclimatization.
One hundred percent of the plantlets survived and grew well in the green house.
Single-node segments from six-day-old seedlings were used as explants and cultured on modified MS (Murashige and Skoog, 1962) medium.
The addition of 0.01 – 1.0 µM indole acetic acid (IAA) in combination with 0.1 – 1.0 µM benzyl adenine (BA) induced axillary shoot proliferation.
During the first two-weeks in culture, 4-15 shoots per explant were proliferated.
The highest number of shoots was obtained from single-node explants cultured on medium supplemented with 0.1 µM IAA and 0.5 µM BA. After three-weeks in culture, up to 23 shoots per explant were produced.
Lengthening the period of culture produced no significant increase in shoot proliferation.
In the last week of a 4-week period of culture, the shoot multiplication rate was very low.
Regenerated shoots were elongated on MS medium without growth regulators.
Shoots were rooted on soil during the period of acclimatization.
One hundred percent of the plantlets survived and grew well in the green house.
Authors
D. Sasmitamihardja, J.S. Hadisutanto, S.N. Widiyanto
Keywords
Albizia falcataria (L.) Fosberg, micropropagation, shoot multiplication
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