Articles
APPLICATION OF TISSUE CULTURE TECHNIQUES IN WOODY SPECIES CONSERVATION, IMPROVEMENT AND DEVELOPMENT IN VIETNAM: AGARWOOD (AQUILARIA CRASSNA PIERRE EX LECOMTE) VIA SHOOT-TIP CULTURE
Article number
692_3
Pages
37 – 42
Language
English
Abstract
Aquilaria crassa (agarwood), a Vietnamese forest tree, was micropropagated using shoot explants from 20-year old trees known to produce the valuable exudates tok. Either shoot tips or internodes could be used for the initial explants, but in subcultures best results were obtained from internodes.
Woody Plant Medium was a better basal medium than Murashige and Skoog, and for initial shoot induction BA at 1mg/l and coconut water at 10% was used. For subcultures, BA at 0.1mg/l, NAA 0.1mg/l and coconut water at 10% gave highest shoot multiplication.
A low level of rooting was obtained using either IBA or NAA at 0.3 mg/l.
Plants transferred to the field grew to 2m after 18 months and had normal morphology.
Woody Plant Medium was a better basal medium than Murashige and Skoog, and for initial shoot induction BA at 1mg/l and coconut water at 10% was used. For subcultures, BA at 0.1mg/l, NAA 0.1mg/l and coconut water at 10% gave highest shoot multiplication.
A low level of rooting was obtained using either IBA or NAA at 0.3 mg/l.
Plants transferred to the field grew to 2m after 18 months and had normal morphology.
Authors
T. Van Minh
Keywords
Agarwood, satalum, resin, micropropagation, tok
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