Articles
MICROPROPAGATION OF LEUCADENDRON
Article number
716_2
Pages
25 – 34
Language
English
Abstract
A collaborative Leucadendron breeding program based at The University of Western Australia has produced thousands of Leucadendron hybrids which are currently being trialled.
Tissue culture techniques are being investigated as a possible method of quickly producing commercial quantities of plants from the limited amount of material available for any new selection.
Multiplication of Leucadendron hybrids has been achieved on MS medium containing 20 g L-1 sucrose and 3 g L-1 Phytogel.
We found that the multiplication rate varied between genotypes but in general increasing the concentration of 6-benzylaminopurine (BAP) in the media from 0.025 mg L-1 to 1 mg L-1 significantly increases the number of shoots produced but decreases their length.
BAP at 0.1 mg L-1 gave the highest number of useful shoots for the three hybrids tested.
Rooting percentages were dependent on genotype and other treatment.
The four genotypes tested were successfully rooted and deflasked by dipping microcuttings in Clonex Purple® (3 g L-1 indole-butyric acid (IBA)) and striking in propagation mix or Growool®. Culturing shoots on media containing IBA at 2 mg L-1 resulted in 0 to 100% of shoots forming roots in vitro depending on genotype while transferring plantlets from this media to propagation mix after 5 weeks resulted in 16.7 to 99.4% survival 6 weeks later.
Root systems formed in propagation mix were superior in terms of root number and length to those formed in Growool® or agar.
It was also shown that rootstrike and deflasking could occur simultaneously in a glasshouse.
Tissue culture techniques are being investigated as a possible method of quickly producing commercial quantities of plants from the limited amount of material available for any new selection.
Multiplication of Leucadendron hybrids has been achieved on MS medium containing 20 g L-1 sucrose and 3 g L-1 Phytogel.
We found that the multiplication rate varied between genotypes but in general increasing the concentration of 6-benzylaminopurine (BAP) in the media from 0.025 mg L-1 to 1 mg L-1 significantly increases the number of shoots produced but decreases their length.
BAP at 0.1 mg L-1 gave the highest number of useful shoots for the three hybrids tested.
Rooting percentages were dependent on genotype and other treatment.
The four genotypes tested were successfully rooted and deflasked by dipping microcuttings in Clonex Purple® (3 g L-1 indole-butyric acid (IBA)) and striking in propagation mix or Growool®. Culturing shoots on media containing IBA at 2 mg L-1 resulted in 0 to 100% of shoots forming roots in vitro depending on genotype while transferring plantlets from this media to propagation mix after 5 weeks resulted in 16.7 to 99.4% survival 6 weeks later.
Root systems formed in propagation mix were superior in terms of root number and length to those formed in Growool® or agar.
It was also shown that rootstrike and deflasking could occur simultaneously in a glasshouse.
Publication
Authors
B. Croxford, G. Yan, R. Sedsley
Keywords
micropropagation, Leucadendron, Proteaceae, multiplication, rootstrike, deflasking, plant growth regulators
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