Articles
FRUIT QUALITY CHARACTERISTICS OF ‘GALIA’ F1 HYBRID (CUCUMIS MELO RETICULATES GROUP) MUSKMELON DEVELOPED FROM A TRANSGENIC MALE PARENT
Article number
731_2
Pages
31 – 38
Language
English
Abstract
Previous work has resulted in transformed Galia male parental lines with an antisense ACC oxidase (CMACO-1) gene that inhibits the last step in ethylene biosynthesis.
From this work, two transgenic male parental lines were developed.
The transformed male lines tested PCR positive for the transgene, but were unselected for delayed ripening.
Transgenic Galia F1 hybrids (TGMH-AS-1 and TGMH-AS-2) were developed from crossing the two transformed male lines to a wild-type female parental line through traditional breeding methods.
During spring and fall 2004, transgenic Galia, wild-type Galia and Gal-52 were grown in a passive-ventilated greenhouse in Citra, Florida.
In spring 2004, no significant differences were observed between transgenic and wild-type Galia fruit harvested fresh or after five days storage at 20°C. Galia is highly susceptible to powdery mildew (Sphaerotheca fuliginea), which was unable to be controlled in spring 2004. Consequently, powdery mildew created significant stress on the plants and potentially negated any positive effect of the transgene.
In fall, powdery mildew was controlled by fungicides.
Fruit from the transgenic lines remained on the vine an average of five days longer than the wild-type.
At full-slip stage, there were no differences in quality parameters (weight, length, width, color, soluble solids content and firmness) between the wild-type and transgenic Galia fruits.
Thus, the superior fruit quality, common of the Galia F1 hybrid is not negatively affected by the transgene.
From this work, two transgenic male parental lines were developed.
The transformed male lines tested PCR positive for the transgene, but were unselected for delayed ripening.
Transgenic Galia F1 hybrids (TGMH-AS-1 and TGMH-AS-2) were developed from crossing the two transformed male lines to a wild-type female parental line through traditional breeding methods.
During spring and fall 2004, transgenic Galia, wild-type Galia and Gal-52 were grown in a passive-ventilated greenhouse in Citra, Florida.
In spring 2004, no significant differences were observed between transgenic and wild-type Galia fruit harvested fresh or after five days storage at 20°C. Galia is highly susceptible to powdery mildew (Sphaerotheca fuliginea), which was unable to be controlled in spring 2004. Consequently, powdery mildew created significant stress on the plants and potentially negated any positive effect of the transgene.
In fall, powdery mildew was controlled by fungicides.
Fruit from the transgenic lines remained on the vine an average of five days longer than the wild-type.
At full-slip stage, there were no differences in quality parameters (weight, length, width, color, soluble solids content and firmness) between the wild-type and transgenic Galia fruits.
Thus, the superior fruit quality, common of the Galia F1 hybrid is not negatively affected by the transgene.
Publication
Authors
J.M. Mitchell, D.J. Cantliffe, H.J. Klee, S.A. Sargent, P.J. Stoffella
Keywords
postharvest, antisense, ACO
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