Home > III International Symposium on Cucurbits > DEVELOPMENT OF A TRIVALENT TRANSGENIC WATERMELON BH-1 RESISTANT TO VIRUS INFECTIONS

Articles

DEVELOPMENT OF A TRIVALENT TRANSGENIC WATERMELON BH-1 RESISTANT TO VIRUS INFECTIONS

Article number

731_8

Pages

71 – 76

Language

English

Abstract

By using the coat protein gene of WMV-2, and replicase genes of ZYMV and CMV, a trivalent expression vector was constructed for transformation of watermelon plants with Agrobacterium tumefaciens. The transgenes were confirmed in progenies of T1, T2 and T3 generations by PCR-Southern blot analysis.
By mechanical inoculation in greenhouse and field trial, the transgenic lines showed resistance to virus infections in relatively high level and the resistant transgenic line BH-1 was selected successfully.

Publication

III International Symposium on Cucurbits

Authors

N. Sheng-Niao, H. Xue-Sen, W. Sek-Man, Y. Jia-Lin, Z. Fu-xin, W. Sen-you, Z. Guang-Ming, S. Fan-Sheng, C. Xiao-Yu, C. Yong-Gang, X. Yan, Z. Fang, W. Xiao-Hong

Keywords

watermelon, virus diseases, gene transformation, resistance to virus infection

Full text

https://doi.org/10.17660/ActaHortic.2007.731.8

Online Articles (72)

1. THE ECP/GR CUCURBITS WORKING GROUP

M.J. Díez | F. Nuez | L. Maggioni | W. van Dooijeweert

2. FRUIT QUALITY CHARACTERISTICS OF ‘GALIA’ F₁ HYBRID (CUCUMIS MELO RETICULATES GROUP) MUSKMELON DEVELOPED FROM A TRANSGENIC MALE PARENT

J.M. Mitchell | D.J. Cantliffe | H.J. Klee | S.A. Sargent | P.J. Stoffella

3. ADVANCES IN BREEDING MELONS FOR RESISTANCE TO VINE DECLINE

4. THE SPANISH MELON GENOMICS INITIATIVE

5. VEGETABLE SCIENCE INTERNATIONAL NETWORK (VEGINET)

Prem Nath

6. NON-CHEMICAL POSTHARVEST DISINFESTATION TREATMENTS FOR CUCURBITS AGAINST BACTROCERA CUCUMIS (FRENCH) (DIPTERA: TEPHRITIDAE)

E.A. Hall | P.L. Leach | R.A. Kopittke

7. INHERITANCE OF BITTERNESS IN CUCUMBER

G. Xingfang | Z. Shengping | G. Yanmei | X. Caiqing

8. DEVELOPMENT OF A TRIVALENT TRANSGENIC WATERMELON BH-1 RESISTANT TO VIRUS INFECTIONS

9. INTERSPECIFIC HYBRIDIZATION OF C. ANGURIA × C. ZEYHERI, C. SATIVUS × C. MELO AND C. SATIVUS × C. METULIFERUS WITH THE USE OF EMBRYO CULTURES

D. Skálová | M. Dziechciarková | A. Lebeda | B. Navrátilová | E. Křístková

10. VARIABILITY OF NUCLEAR DNA CONTENT AMONG AND WITHIN THREE CUCURBIT GENERA

N. Gasmanová | A. Lebeda | I. Dolezalová | H.S. Paris

11. EFFECT OF GENOTYPE, SOURCE TISSUE AND MEDIA COMPOSITION ON CUCUMIS AND CUCURBITA PROTOPLAST ISOLATION AND REGENERATION

J. Gajdová | B. Navrátilová | J. Smolná | A. Lebeda

12. CLONING OF TRYPTOPHAN MONOXYGENASE GENE IN PSEUDOMONAS SYRINGAE AND TRANSFORMATION INTO CUCUMBER

H. Liu | Z. Qin

13. POLLEN VIABILITY AND GERMINATION RATES OF 45 WATERMELON GENOTYPES

P. Gok | H. Yetisir | I. Solmaz | N. Sari | S. Eti

14. FRUIT CHARACTERIZATION OF SOME TURKISH MELON GENOTYPES

N. Sari | I. Solmaz

15. EVALUATION OF 10 SELECTED STRAINS OF CUCURBITA MOSCHATA DUCH.

J.-T. Gu | X.-C. Zhang | S. Fan

16. FOLIAR APPLIED POTASSIUM: EFFECTS ON CANTALOUPE QUALITY

G. E. Lester | J. L. Jifon

17. WATERMELONS AND HEALTH

P. Perkins-Veazie | J. K. Collins | B. Clevidence

18. OSMOTIC DEHYDRATION OF CANTALOUPE: INFLUENCES OF TIME AND CONCENTRATION

F. Shahidi | F. Amidi | M. Mohebbi | A. Ganjloo

19. EFFECTS OF PLUG SIZE, MYCORRHIZAE INOCULANT AND GROWTH PERIOD ON THE DEVELOPMENT OF WATERMELON TRANSPLANTS

20. SHOOT/ROOT RATIO OF CUCUMBER IS AFFECTED BY ENVIRONMENTAL CONDITIONS

H.-P. Kläring | M.A. Kyuchukova

21. IN VITRO MICROPROPAGATION OF BOTTLE GOURD (LAGENARIA SICERARIA; CUCURBITACEAE): PROSPECTIVE ROOTSTOCKS FOR THE GRAFTING OF WATERMELON AND OTHER CUCURBITS

S. Saha | H. Kazumi

22. THE HISTORY AND PRESENT STATE OF THE GRAFTING OF CUCURBITACEOUS VEGETABLES IN JAPAN

Y. Sakata | T. Ohara | M. Sugiyama

23. MINI TRIPLOID WATERMELON CULTIGEN EVALUATIONS FOR YIELD AND QUALITY, AND MARKETING IN THE UNITED STATES

J. Jonathan R. | Schultheis | W. Bradford Thomps | W. R. Jester

24. INTEGRATION OF SOLAR-HEATING AND SOIL-AMENDMENT, AN EFFECTIVE CONTROL MEASURE AGAINST ROOT-KNOT NEMATODES IN CUCUMBER FIELDS

M. Nasr Esfahani

25. CUCUMBER YIELD UNDER REGULAR DEFICIT IRRIGATION AND MULCHING TREATMENTS

M.N. Nimah

26. THE EFFECTS OF CELL VOLUME AND PRODUCTION PERIODS ON PROFITABILITY OF WATERMELON TRANSPLANTS PRODUCTION

M. Oplanić | D. Ban | A.S. Ilak Persurić | M. Radulović

27. ADVANCING BITTER GOURD CROP BY PLASTIC MULCHING: A PROFITABLE AND SUSTAINABLE TECHNOLOGY FOR PERI-URBAN AREAS OF NORTHERN INDIA

B. Singh | M. Kumar | S.P. Mehto

28. SYSTEMIC ACQUIRED RESISTANCE AS A STRATEGY FOR DISEASE MANAGEMENT IN ROCKMELON (CUCUMIS MELO VAR. RETICULATUS)

R. McConchie | K. McDonald | B. Anwaral | S.C. Morris

29. MANAGING CUCURBIT POWDERY MILDEW AND FUNGICIDE RESISTANCE

M. T. McGrath

30. A PRELIMINARY APPROACH TO IDENTIFY THE PHYSIOLOGICAL SUBSTRATES OF PUMPKIN GLUTATHIONE S-TRANSFERASE THROUGH INHIBITION STUDIES

M.D. Hossain | M. Fujita | T. Suzuki

31. HYDROPONIC GREENHOUSE PRODUCTION OF SPECIALTY CUCURBIT CROPS

Daniel J. Cantliffe | Nicole L. Shaw | Juan C. Rodriguez | Peter J. Stoffella

32. COMPARATIVE WATER AND FERTILIZER USE EFFICIENCIES OF TWO PRODUCTION SYSTEMS FOR CUCUMBERS

E. Jovicich | D. J. Cantliffe | E. H. Simonne | P. J. Stoffella

33. EFFECT OF LIGHT QUALITY ON THE GROWTH AND PHOTOSYNTHETIC CHARACTERISTICS OF CUCUMBER CUCUMIS SATIVUS L. UNDER SOLAR GREENHOUSE

S. Wang | S. Fan | Y. Kong | C. Qingjun

34. A NEW CROP FOR NORTH AMERICAN GREENHOUSE GROWERS: BEIT ALPHA CUCUMBER – PROGRESS OF PRODUCTION TECHNOLOGY THROUGH UNIVERSITY RESEARCH TRIALS

Nicole L. Shaw | Daniel J. Cantliffe | Peter J. Stoffella

35. ALTERNATIVE USE OF PINE BARK MEDIA FOR HYDROPONIC PRODUCTION OF ‘GALIA’ MUSKMELON RESULTS IN PROFITABLE RETURNS

N. L. Shaw | D. J. Cantliffe | J. C. Rodriguez | Z. Karchi

36. PROTECTED CULTIVATION OF CUCURBITS UNDER LOW-COST PROTECTED STRUCTURES: A SUSTAINABLE TECHNOLOGY FOR PERI-URBAN AREAS OF NORTHERN INDIA

B. Singh | M. Kumar | N.P.S. Sirohi

37. EFFECT OF NITROGEN FORM ON YIELD, FRUIT NUMBER AND LEAF NUTRIENT CONTENT OF TWO GREENHOUSE CUCUMBER CULTIVARS IN SOILLESS CULTURE

F. Soltani | A. Kashi | M. Babalar

38. TECHNO-ECONOMIC FEASIBILITY OF YEAR-ROUND PARTHENOCARPIC CUCUMBER CULTIVATION UNDER NATURALLY VENTILATED GREENHOUSE IN NORTHERN INDIA

B. Singh | M. Kumar | N.P.S. Sirohi

39. ANALYSING INDUSTRY NEEDS TO FINE TUNE IPM PROJECT ACTIVITIES

S. Heisswolf | I. Kay

40. EFFECT OF ROW COVERS ON SILVERLEAF WHITEFLY (HOMOPTERA: ALEYRODIDAE) POPULATION DENSITIES AND CROP YIELD IN ZUCCHINI

41. THE MANAGEMENT OF THE MAJOR INSECT PESTS BACTOCERA CUCURBITAE (DIPTERA: TEPHRITIDAE) AND AULACAPHORA SPP. (COLEOPTERA: SCARABAEIDAE) IN CUCURBITS IN SOUTHERN SRI LANKA UNDER THREE INTENSIVE SYSTEMS: INTEGRATED, CHEMICAL AND ORGANIC AGRICULTURE

R. Rajapakse | D. Ratnasekera

42. CHALLENGES FOR THE IMPLEMENTATION OF INTEGRATED PEST MANAGEMENT OF CUCUMBER PESTS IN PROTECTED CROPS – AN AUSTRALIAN PERSPECTIVE

M.Y. Steiner | S. Goodwin

43. EPIDEMIOLOGY OF DAMPING-OFF DISEASE IN GREENHOUSE CUCUMBER CROPS IN THE SULTANATE OF OMAN

44. TEMPORAL CHANGES IN PATHOGENICITY AND FUNGICIDE RESISTANCE IN PSEUDOPERONOSPORA CUBENSIS POPULATIONS

A. Lebeda | J. Urban

45. TEMPORAL AND SPATIAL DYNAMICS OF POWDERY MILDEW SPECIES ON CUCURBITS IN THE CZECH REPUBLIC

E. Křístková | A. Lebeda | B. Sedláková

46. LATE-SEASON VINE DECLINES OF MELONS: PATHOLOGICAL, CULTURAL OR BOTH?

R. D. Martyn

47. COMPARATIVE PERFORMANCE AND PRESERVATION OF CHEMICAL MANAGEMENT TOOLS FOR POWDERY MILDEW ON MUSKMELON

M.E. Matheron | M. Porchas

48. PSEUDOMONAS VIRIDIFLAVA: A NEW BACTERIAL PATHOGEN OF MELON AND WATERMELON IN TURKEY

Y. Aysan | M. Mirik | K. Abak | M. Yildiz | F. Sahin

49. BIOFUMIGATION FOR THE MANAGEMENT OF DAMPING-OFF IN GREENHOUSE CUCUMBERS IN THE SULTANATE OF OMAN

M. Deadman | Y. Al Maqbali | A. Al Sa'di | H. Al Hasani | M. Al Nabhani

50. SALINITY AND PYTHIUM APHANIDERMATUM-INDUCED DAMPING-OFF DISEASE OF GREENHOUSE CUCUMBERS IN OMAN

51. ANTIMICROBIAL EFFICACY OF SEED EXTRACTS OF CUCURBITA PEPO

M. Singhal

52. SUDDEN COLLAPSE OF MUSKMELON – A MAJOR NEW DISEASE CONSTRAINT IN OMAN

M. Deadman | I.A. Khan | A. Al Sa'di | M. Al Nabhani | Y. Al Maqbali

53. TEMPORAL CHANGES IN PATHOGENICITY STRUCTURE OF CUCURBIT POWDERY MILDEW POPULATIONS

A. Lebeda | B. Sedláková | E. Křístková

54. FLOWER AND FRUIT ROT OF GREENHOUSE CUCUMBER CROPS IN THE SULTANATE OF OMAN

M. Deadman | Y. Al Maqbali | A. Al Sa'di | S. Al Kaabi | K. Al Kiyoomi

55. ASSESSING POTENTIAL BENEFITS OF TECHNOLOGIES: BENEFITS OF IMPROVING POSTHARVEST QUALITY TECHNOLOGIES IN THE AUSTRALIAN ROCKMELON INDUSTRY

S. Wei | R. Maczkowiack | R. McConchie

56. MOLECULAR CONTROL OF FRUIT RIPENING AND SENSORY QUALITY OF CHARENTAIS MELON

D. Manriquez | F.B. Flores | I. El-Sharkawy | A. Latché | J.C. Pech

57. A SINGLE INSERTION OF ACC OXIDASE GENE IN ANTISENSE ORIENTATION EXTENDS THE SHELF LIFE IN MUSKMELON ‘GALIA’ HYBRID PARENTAL LINE (CUCUMIS MELO L. VAR. RETICULATUS SER.)

H.G. Nunez-Palenius | H.J. Klee | D.J. Huber | D.J. Cantliffe

58. ISOLATION AND CHARACTERIZATION OF AN ETHYLENE RECEPTOR HOMOLOG GENE, CM-ETR2, FROM MELON FRUIT

W. O. Owino | T. Shoji | H. Ezura

59. WATERMELON GENETIC RESOURCES IN TURKEY AND THEIR CHARACTERISTICS

N. Sari | I. Solmaz | H. Yetisir | H. Unlu

60. POSTHARVEST HARPIN TREATMENT SUPPRESSES DECAY AND INDUCES THE ACCUMULATION OF DEFENSE-RELATED ENZYMES IN HAMI MELONS

B. Yang | G. Yonghong | G. Yurong | Z. Jie

61. CHANGES OF QUALITY PARAMETERS IN WATERMELON DURING STORAGE

M. Radulović | D. Ban | B. Sladonja | V. Lusetić-Bursić

62. OSMOTIC TREATMENTS IN MELON (CANTALOUPE AND MUSKMELON) PROCESSING: PHYSICOCHEMICAL AND ORGANOLEPTICAL EFFECTS

F. Shahidi | A. Ganjloo | M. Mohebbi

63. A PERCEPTION TO OSMODEHYDROFREEZING: A NOVEL APPROACH IN CANTALOUPE PROCESSING

F. Shahidi | F. Amidi | M. Mohebbi | A. Kouchaki

64. EFFECT OF CALCIUM CHLORIDE SOLUTION ON PHYSIOLOGICAL CHANGES OF ‘SUN LADY’ CANTALOUPE DURING STORAGE

P. Sa-ngunanwongwichit | C. Wongs-Aree | S. Kanlayanarat

65. CURRENT TRENDS IN CUCURBIT PRODUCTION IN THE U.S.

D. J. Cantliffe | N. L. Shaw | P. J. Stoffella

66. CUCURBIT PRODUCTION IN AUSTRALIA

G. Kelly

67. CUCURBITS – EVERYONE’S CROP

Prem Nath

68. MELON PRODUCTION IN CHINA

B. Yang | G. Yonghong | W. Chunling | L. Xuewen

69. THE EUROPEAN CENTRAL CUCURBITS DATABASE (ECCUDB)

M.J. Díez | A. Gil | F. Nuez

70. SUCCESSES AND LIMITATIONS OF SOME CUCURBIT RELEASES IN QUEENSLAND

M.E. Herrington

71. STABILITY OF RESISTANCE TO PAPAYA RINGSPOT VIRUS IN MELON (CUCUMIS MELO) AND PUMPKIN (CUCURBITA SPP.)

M.E. Herrington | D.M. Persley

72. INFLUENCE OF FERTIGATION RATES APPLIED AT DIFFERENT DEVELOPMENTAL STAGES ON MUSKMELON EARLINESS, YIELD, AND QUALITY

R.L. Hassell | T.L. Phillips | R.J. Dufault