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Articles

ISOLATION AND OBSERVATION OF VIABLE SPERM CELLS FROM THE POLLENS OF LOQUAT

Article number
750_29
Pages
193 – 196
Language
English
Abstract
In this study, the modified osmotic shock technique and density gradient centrifugation technique were used to isolate and purify the sperm cells of loquat cv. ‘Dawuxing’. We observed the vegetative and reproductive cells in pollen tube with the use of DNA specific fluochrome 4′,6-diamidino-2-phenylindole (DAPI) and methyl salicylate.
The movement and release of sperm cells in pollen tube were observed systematically.
Living sperm cells were obtained at last, whose density was 4.8 × 106 each milliliter.
And the survival rate of them was 54% tested by 0.4% Evans blue.

Publication
II International Symposium on Loquat
Authors
L.Y. Zhang, Q.G. Guo, G.L. Liang
Keywords
loquat, sperm cell, pollen, isolation
Full text
https://doi.org/10.17660/ActaHortic.2007.750.29
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