Articles
GENETIC PURITY TEST OF F1 HYBRID TOMATO USING MOLECULAR MARKER ANALYSIS
Article number
771_34
Pages
231 – 238
Language
English
Abstract
Three DNA molecular marker systems, RAPD, ISSR and SSR, were used to test seed genetic purity of a hybrid tomato Hezuo906. Genetic relationships of the F1 hybrid and their two parents were analyzed with 218 RAPD random decamer primers, 54 ISSR primers, and 49 SSR primers.
Among the 321 primers screened, two dominant RAPD primers and two codominant SSR primers were selected for testing the genetic purity of the F1 seeds.
A total of 208 hybrid individuals were investigated with the selected primers.
The combined results of the marker analyses confirmed that ten of 208 Hezuo906 were false hybrids, and the overall genetic purity of the F1 hybrid seed lot was 95.1%. This study showed that the molecular markers are highly efficient and reproducible for genetic purity testing of tomato commercial hybrid seeds.
Among the 321 primers screened, two dominant RAPD primers and two codominant SSR primers were selected for testing the genetic purity of the F1 seeds.
A total of 208 hybrid individuals were investigated with the selected primers.
The combined results of the marker analyses confirmed that ten of 208 Hezuo906 were false hybrids, and the overall genetic purity of the F1 hybrid seed lot was 95.1%. This study showed that the molecular markers are highly efficient and reproducible for genetic purity testing of tomato commercial hybrid seeds.
Authors
L. Liu, Y. Wang, Y. Gong, X. Zhai, F. Yu, H. Shen
Keywords
‘Hezuo906’, RAPD, SSR, Solanum lycopersicon
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