Articles
STUDY ON THE ROOTING OF GERBERA JAMESONII IN VITRO IN MEDIA WITHOUT AGAR
Article number
771_35
Pages
239 – 242
Language
English
Abstract
There are two tough problems in tissue culture of plants.
One is high cost, another is low survival rate of transplant.
In order to reduce the cost of tissue culture with agar, and to increase survival rate of transplant, another kind of support in culture medium instead of agar is needed, which is cheap and can be washed down easily.
We respectively put the buds of Gerbera jamesonii in nutrient solution (MS+BA 15 mgL-1 +IBA 0.2 mgL-1) with three kinds of medium in vitro to induce to root, which are grit, sponge, and agar.
And then rooted plantlets were transplanted from culture bottle to flower bed.
At the same time, rooting rate and survival rate of transplant were counted.
Results were showed: the rooting rate was highest in nutrient solution with grit (which were respectively 96.55, 89.47, 94.11% with grit, sponge, and agar), and the panorama of growth was also best; Furthermore, its survival rate of transplant was highest (which were respectively 97.63, 96.25, 78.18% with grit, sponge, and agar). Moreover, there were some details affecting the results such as the volume of liquid, and the way to treat support materials.
The success in the rooting culture of Gerbera jamesonii without agar showed that grit and sponge could be used instead of agar.
If the technique can be widely used in tissue culture of other plants, it will be great meaningful in the field of plants.
One is high cost, another is low survival rate of transplant.
In order to reduce the cost of tissue culture with agar, and to increase survival rate of transplant, another kind of support in culture medium instead of agar is needed, which is cheap and can be washed down easily.
We respectively put the buds of Gerbera jamesonii in nutrient solution (MS+BA 15 mgL-1 +IBA 0.2 mgL-1) with three kinds of medium in vitro to induce to root, which are grit, sponge, and agar.
And then rooted plantlets were transplanted from culture bottle to flower bed.
At the same time, rooting rate and survival rate of transplant were counted.
Results were showed: the rooting rate was highest in nutrient solution with grit (which were respectively 96.55, 89.47, 94.11% with grit, sponge, and agar), and the panorama of growth was also best; Furthermore, its survival rate of transplant was highest (which were respectively 97.63, 96.25, 78.18% with grit, sponge, and agar). Moreover, there were some details affecting the results such as the volume of liquid, and the way to treat support materials.
The success in the rooting culture of Gerbera jamesonii without agar showed that grit and sponge could be used instead of agar.
If the technique can be widely used in tissue culture of other plants, it will be great meaningful in the field of plants.
Authors
X.-H. Wang, X.-F. Tan, F.-M. Hu
Keywords
tissue culture, non-organic medium, Gerbera jamesonii, survival rate of transplant, seedling
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