Articles
PRELIMINARY RESULTS OF TRIALS EVALUATING THE BEHAVIOUR TO PLUM POX VIRUS OF DIFFERENT APRICOT CROSSES
Article number
781_44
Pages
295 – 303
Language
English
Abstract
This work reports the preliminary results of Plum pox virus (PPV) infection on apricot seedlings belonging to two different crosses from a breeding program of the Universities of Milan and Bologna (Italy): Lito × BO 81604311 (121 seedlings) and Harcot × Reale dImola (116).
An improved protocol developed by the Department of Plant Protection and Applied Microbiology of University of Bari was applied.
The sequential steps of the protocol are: 1) bud grafting onto myrabolan and Prunus persica GF305; 2) chip-bud inoculation on the above rootstocks with known isolates of PPV-M and PPV-D (both strains for the first cross and only D-strain for the second one); 3) visual inspections on rootstock and apricot for leaf symptoms expression: during each growth cycle leaf symptoms were scored from 0 (no symptoms) to 3 (higher intensity of symptoms); and 4) testing by ELISA and IC-RT-PCR. The protocols provides for the observation of the seedlings until the fruiting stage.
The results after two vegetative cycles since the inoculation demonstrate: a) most of the seedlings of the cross Harcot × Reale dImola showed symptoms and were ELISA-positive since the first controls (78% and 42% in presence, respectively for PPV-M and PPV-D strain); b) most of the seedlings belonging to the cross Lito × BO 81604311, in the first vegetative cycle after inoculation (2005), showed symptoms since the earliest visual inspection and were ELISA-positive for both strains (92% and 73% in presence, respectively for PPV-M and PPV-D strain). In springtime 2006 a greater number of seedlings, infected with PPV-M and PPV-D strains, were symptomless and ELISA-negative; the myrabolan revealed to be an effective indicator, showing symptoms since the first visual inspections after two-three weeks since sprouting.
An improved protocol developed by the Department of Plant Protection and Applied Microbiology of University of Bari was applied.
The sequential steps of the protocol are: 1) bud grafting onto myrabolan and Prunus persica GF305; 2) chip-bud inoculation on the above rootstocks with known isolates of PPV-M and PPV-D (both strains for the first cross and only D-strain for the second one); 3) visual inspections on rootstock and apricot for leaf symptoms expression: during each growth cycle leaf symptoms were scored from 0 (no symptoms) to 3 (higher intensity of symptoms); and 4) testing by ELISA and IC-RT-PCR. The protocols provides for the observation of the seedlings until the fruiting stage.
The results after two vegetative cycles since the inoculation demonstrate: a) most of the seedlings of the cross Harcot × Reale dImola showed symptoms and were ELISA-positive since the first controls (78% and 42% in presence, respectively for PPV-M and PPV-D strain); b) most of the seedlings belonging to the cross Lito × BO 81604311, in the first vegetative cycle after inoculation (2005), showed symptoms since the earliest visual inspection and were ELISA-positive for both strains (92% and 73% in presence, respectively for PPV-M and PPV-D strain). In springtime 2006 a greater number of seedlings, infected with PPV-M and PPV-D strains, were symptomless and ELISA-negative; the myrabolan revealed to be an effective indicator, showing symptoms since the first visual inspections after two-three weeks since sprouting.
Publication
Authors
A. Bazzoni, A. Didonna, V. Savino, F. Palmisano
Keywords
sharka, Prunus spp., resistance
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