Home > XX International Symposium on Virus and Virus-Like Diseases of Temperate Fruit Crops – Fruit Tree Diseases > DETECTION AND CHARACTERIZATION OF SOME ISOLATES OF CHERRY GREEN RING MOTTLE VIRUS

Articles

DETECTION AND CHARACTERIZATION OF SOME ISOLATES OF CHERRY GREEN RING MOTTLE VIRUS

Article number

781_7

Pages

65 – 70

Language

English

Abstract

A one-step reverse transcription-polymerase chain reaction (RT-PCR) method was applied for detection of Cherry green ring mottle virus (CGRMV) in naturally infected sweet and sour cherry trees and in woody indicators.
Virus-specific primers and degenerate primers were used to amplify viral genomic fragments of selected CGRMV isolates.
Six isolates of CGRMV were analysed by a primer combination encompassing the coat protein gene.
The amplification products were cloned and sequenced.
Nucleotide sequences of the coat protein of virus isolates were compared to corresponding regions of published sequences of CGRMV. On the basis of the phylogenetic analysis of the nucleotide sequences of the coat protein gene all studied isolates weregrouped in clade II. All investigated CGRMV isolates induced symptoms on woody indicators Prunus serrulata Shirofugen and Kwanzan cvs.

Publication

XX International Symposium on Virus and Virus-Like Diseases of Temperate Fruit Crops – Fruit Tree Diseases

Authors

B. Komorowska, M. Cieślińska

Keywords

cherry, RT-PCR, sequencing, phylogenetic analysis

Full text

https://doi.org/10.17660/ActaHortic.2008.781.7

Online Articles (83)

1. SILENCING IN PRUNUS: A NATURAL DEFENSE DEVELOPED BY WOODY FRUIT TREES IN RESPONSE TO VIRUS INFECTION

2. PRUNUS SPİNOSA L.-A NATURAL WİLD HOST OF SOME IMPORTANT FRUİT VİRUSES İN TEKİRDAĞ, TURKEY

H. İlbağı | A. Çıtır | H. Bostan

3. GENOMIC DIVERSITY OF CHERRY CAPILLOVIRUS A (CVA) AND SUITABILITY OF VARIOUS ASSAYS FOR ITS DETECTION

4. PRODUCTION OF FULL LENGTH COPIES OF THE GENOMIC RNAS OF PRUNUS NECROTIC RINGSPOT VIRUS

S.W. Scott | M.T. Zimmerman

5. INVESTIGATION ON OCCURRENCE OF TRICHO-, FOVEA- AND CAPILLOVIRUSES IN ANCIENT FRUIT TREE CULTIVARS IN CAMPANIA

M. Barone | D. Alioto | A. Ragozzino | T. Candresse

6. DETECTION OF THE VIRAL DISEASES PRESENTLY WITH THE STONE FRUIT SPECIES IN ROMANIA

M. Isac | C. Plopa | M. Calinescu | A. Myrta

7. DETECTION AND CHARACTERIZATION OF SOME ISOLATES OF CHERRY GREEN RING MOTTLE VIRUS

B. Komorowska | M. Cieślińska

8. VIRUSES OF STONE FRUITS IN BOSNIA AND HERZEGOVINA

S. Matić | M. Al Rwahnih | A. Myrta | G. Đurić

9. APRICOT LATENT VIRUS STUDIES IN THE CZECH REPUBLIC

L. Grimova | M. Zouhar | P. Rysanek

10. THE EFFECT OF GRADIENT TEMPERATURE PATTERN ON ANNEALING EFFICIENCY

M. Ocak | H.M. Sipahioğlu | M. Usta

11. VIRUS INFECTIONS IN STONE FRUIT TREES FROM DIFFERENT PROVINCES OF TURKEY

Çiğdem Ulubaş Serçe | Filiz Ertunc

12. THERMOTHERAPY OF GRAPEVINES AND APRICOTS BY REASON OF VIRUSES AND PHYTOPLASMA ELIMINATION

13. DETECTION OF SOME VIRUSES OF STONE FRUITS IN PURPLE LEAF PLUM (PRUNUS CERASIFERA) AS ORNAMENTAL PLANT IN THE EASTERN MEDITERRANEAN REGION OF TURKEY

G. Sertkaya

14. SEROLOGICAL IDENTIFICATION OF SOME IMPORTANT VIRUSES ON FRUIT TREES AND BUSHES IN TEKIRDAĞ PROVINCE OF TURKEY

A. Çıtır | H. İlbağı

15. DEVELOPMENT OF ROUTINE DUPLEX RT-PCR TESTS FOR CERTIFICATION OF FRUIT TREE MULTIPLICATION MATERIAL

S. Massart | S. Roussel | J. Kummert | O. Dutrecq | M.H. Jijakli

16. DETECTION AND DISTRIBUTION OF FOUR POME FRUIT VIRUSES IN GERMPLASM COLLECTION IN THE CZECH REPUBLIC

M. Hassan | J. Polák | F. Paprštein

17. EFFECT OF APPLE CHLOROTIC LEAF SPOT VIRUS ON YIELD AND QUALITY OF FRUITS FROM ‘GOLDEN DELICIOUS’ AND ‘SAMPION’ APPLE TREES

M. Cieślińska | K.P. Rutkowski

18. A PRELIMINARY ACCOUNT ON APPLE VIRUSES IN MEDITERRANEAN REGION OF TURKEY

N. Birişik | A. Myrta | M. Hassan | S. Baloğlu

19. DISTRIBUTION OF APPLE MOSAIC ILARVIRUS (APMV) IN TURKEY

S. Dursunoglu | F. Ertunc

20. DETECTION, DISTRIBUTION AND GENETIC DIVERSITIES OF APPLE STEM PITTING VIRUS AND APPLE STEM GROOVING VIRUS IN THE CZECH REPUBLIC

J.K. Kundu

21. DETERMINATION OF VIRUSES IN IMPORTED FRUIT PROPAGATION MATERIALS

S. Özdemir | A. Kaya

22. COMPARATIVE RESULTS OF DETECTION OF POME FRUIT VIRUSES BY DIFFERENT METHODS

S. Paunović | D. Jevremović

23. RT-PCR DETECTION OF APPLE MOSAIC VIRUS INFECTION IN SOME WEED HOSTS FOUND IN HAZELNUT ORCHARDS IN TURKEY

M. Arli Sokmen | N.D. Kutluk Yilmaz | H. Mennan | M.A. Sevik

24. FIRST REPORT OF APPLE CHLOROTIC LEAF SPOT VIRUS IN QUINCE (CYDONIA OBLONGA MILL.) IN TURKEY

B. Akbaş | D. İlhan

25. THE INCIDENCE AND STRAIN VARIATION OF APPLE STEM GROOVING AND APPLE STEM PITTING VIRUSES IN AUSTRALIAN POME FRUIT

B.C. Rodoni | F.E. Constable

26. PLUM POX VIRUS DISEASE IN TURKEY

S. Sahtiyancı

27. PROPOSAL FOR A NEW INTERNATIONAL PROTOCOL FOR DETECTION AND IDENTIFICATION OF PLUM POX VIRUS: VALIDATION OF THE TECHNIQUES

28. OCCURRENCE AND DISTRIBUTION OF PLUM POX VIRUS IN CROATIA

I. Mikec | V. Kajić | M. Krajačić | D. korić

29. OCCURRENCE AND CONTROL OF PLUM POX VIRUS IN THE NETHERLANDS

J.T.J. Verhoeven | J.W. Roenhorst | G.P. Jongedijk

30. THE PARTIAL MOLECULAR CHARACTERIZATION OF PLUM POX VIRUS INFECTING SWEET CHERRY TREES IN THE CZECH REPUBLIC

31. ISOTHERMAL AMPLIFICATION FOR DETECTION OF PLUM POX VIRUS

A. Olmos | E. Bertolini | M. Cambra

32. SPOT REAL-TIME RT-PCR: A METHOD FOR DIRECT DETECTION OF PLUM POX VIRUS AVOIDING RNA EXTRACTION

33. COMPARATIVE TECHNIQUES TO PERFORM KOCH’S POSTULATES WITH PLUM POX VIRUS

M. Monsion | P. Briard | M. Glasa | M. Ravelonandro

34. PREVALENCE AND GENETIC STRUCTURE OF PPV-M IN SIX EUROPEAN COUNTRIES

35. SPATIAL PATTERN AND GENETIC STRUCTURE OF PPV-M IN A DELIMITED AREA OF STONE FRUIT ORCHARDS IN SOUTHERN FRANCE

S. Dallot | M. Boeglin | G. Labonne

36. DETECTION AND PARTIAL MOLECULAR CHARACTERIZATION OF PPV RECOMBINANT ISOLATES IN LOCALITY

S. Gadiou | D. afárová | M. Navrátil

37. PRELIMINARY BIOLOGICAL AND MOLECULAR CHARACTERIZATION OF MILD PPV ISOLATES NATURALLY INDUCED IN N. BENTHAMIANA PLANTS

C. Varveri | N. Vassilakos

38. TESTING THE SPATIAL ASSOCIATION OF DISEASE PATTERNS BETWEEN TWO DATES IN ORCHARDS

39. PROBLEMS IN THE DETERMINATION OF INHERITANCE OF PLUM POX VIRUS RESISTANCE IN APRICOT

G. Llácer | M.L. Badenes | C. Romero

40. THE DETERMINATION OF SOURCES OF RESISTANCE TO PLUM POX VIRUS SUITABLE FOR PEACH

J. Polák | I. Oukropec

41. THE HYPERSENSITIVITY OF EUROPEAN PLUM (PRUNUS DOMESTICA L.) AGAINST THE PLUM POX VIRUS

M. Neumüller | W. Hartmann

42. THE ABILITY OF A MILD ISOLATE OF PLUM POX VIRUS TO CROSS-PROTECT AGAINST SHARKA VIRUS

M. Ravelonandro | P. Briard | M. Glasa | S. Adam

43. CHARACTERISATION OF DIFFERENT INTERACTIONS BETWEEN CULTIVARS OF STONE FRUITS AND PLUM POX VIRUS

J. Polák

44. PRELIMINARY RESULTS OF TRIALS EVALUATING THE BEHAVIOUR TO PLUM POX VIRUS OF DIFFERENT APRICOT CROSSES

A. Bazzoni | A. Didonna | V. Savino | F. Palmisano

45. TWO-GENE RESISTANCE TO PLUM POX VIRUS IN APRICOT

J. Salava | J. Polák | B. Krka

46. ENVIRONMENTAL IMPACT ASSESSMENT OF TRANSGENIC PLUMS ON THE DIVERSITY OF PLUM POX VIRUS POPULATIONS

47. LITTLE CHERRY CLOSTEROVIRUSES-1 AND -2, THEIR GENETIC VARIABILITY AND DETECTION BY REAL-TIME-PCR

W. Jelkmann | S. Leible | M. Rott

48. DNA MICROARRAYS: 21^ST CENTURY PATHOGEN DETECTION

A. Hadidi | M. Barba

49. NEW MOLECULAR TYPING TOOLS TO MONITOR FRUIT TREE PHYTOPLASMA VARIABILITY IN THE 16SRX TAXONOMIC GROUP

J.-L. Danet | H. Bahriz | A. Cimerman | X. Foissac

50. PHYTOPLASMAS INFECTING FRUIT TREES IN SERBIA

B. Duduk | M. Ivanović | S. Paltrinieri | A. Bertaccini

51. MOLECULAR IDENTIFICATION OF PHYTOPLASMAS IN CULTIVAR COLLECTION AND PRODUCTION PLANTATIONS OF APPLE AND PEAR TREES IN THE CZECH REPUBLIC

J. Fránová | K. Petrzik | F. Paprtein | J. Kučerová | M. Růičková

52. RESEARCH ON CANDIDATUS PHYTOPLASMA MALI TRANSMISSION BY INSECT VECTORS IN TRENTINO

L. Mattedi | F. Forno | C. Cainelli | M.S. Grando | W. Jarausch

53. ESTABLISHMENT OF AN IN VITRO SCREENING SYSTEM OF APPLE PROLIFERATION-RESISTANT ROOTSTOCK GENOTYPES BASED ON MICROGRAFTING

C. Bisognin | A.M. Ciccotti | M. Moser | M.S. Grando | W. Jarausch

54. DEVELOPMENT OF RESISTANT ROOTSTOCKS FOR THE CONTROL OF APPLE PROLIFERATION DISEASE

W. Jarausch | C. Bisognin | T. Peccerella | B. Schneider | E. Seemüller

55. ADAPTATION OF REAL-TIME PCR ASSAY FOR SPECIFIC DETECTION OF APPLE PROLIFERATION PHYTOPLASMA

M. Aldaghi | S. Massart | S. Roussel | O. Dutrecq | M.H. Jijakli

56. MOLECULAR DIFFERENTIATION OF ‘CANDIDATUS PHYTOPLASMA MALI’ AND ITS SPREADING IN FRIULI VENEZIA GIULIA REGION (NORTH-EAST ITALY)

M. Martini | P. Ermacora | L. Falginella | N. Loi | L. Carraro

57. INFECTIVITY OF CACOPSYLLA PICTA (SYN. CACOPSYLLA COSTALIS), VECTOR OF ‘CANDIDATUS PHYTOPLASMA MALI’ IN NORTH EAST ITALY

L. Carraro | F. Ferrini | P. Ermacora | N. Loi | G. Labonne

58. PHYTOPLASMAS IN DECLINING CHERRY PLANTS

S. Paltrinieri | A. Bertaccini | C. Lugaresi

59. EPIDEMIOLOGY OF EUROPEAN STONE FRUIT YELLOWS IN GERMANY

60. EUROPEAN STONE FRUIT YELLOWS: CONSEQUENCES OF THE LIFE CYCLE OF THE VECTOR AND OF THE MULTIPLICATION OF THE PHYTOPLASMA IN THE INSECT ON THE EPIDEMIOLOGY OF THE DISEASE

G. Thébaud | M. Yvon | G. Labonne | R. Alary

61. IDENTIFICATION OF FRUIT TREE PHYTOPLASMAS AND THEIR VECTORS IN BOSNIA AND HERZEGOVINA

D. Delić | M. Martini | P. Ermacora | L. Carraro | A. Myrta

62. INFLUENCE OF THE TIME OF SAMPLING ON DETECTION OF ESFY PHYTOPLASMA

T. Nečas | B. Krka | V. Maková | M. Adam

63. THE POSSIBILITY OF ESFY PHYTOPLASMA TRANSMISSION: THROUGH FLOWERS AND SEEDS

T. Nečas | V. Maková | B. Krka

64. A PRELIMINARY ACCOUNT OF THE PRESENCE OF PEAR DECLINE IN MARMARA REGION OF TURKEY

K. Cağlayan | C. Ulubaş Serçe | H. Öztürk | N.S. Gencer

65. COMPARISON OF DIFFERENT DETECTION SYSTEMS FOR APPLE PROLIFERATION PHYTOPLASMAS IN TRENTINO (NORTH ITALY)

A. Bertaccini | S. Paltrinieri | M. Martini | P. Ermacora

66. NATURAL AND EXPERIMENTAL TRANSMISSION OF CANDIDATUS PHYTOPLASMA MALI BY ROOT BRIDGES

67. APPLE PROLIFERATION SUSCEPTIBILITY AND SENSITIVENESS IN OLD APPLE-TREES VARIETIES NATURALLY AND ARTIFICIALLY INFECTED

P. Ermacora | L. Carraro | M. Martini | N. Loi | R. Osler

68. MOLECULAR TESTS TO DETERMINE APPLE PROLIFERATION PHYTOPLASMA PRESENCE IN PSYLLID VECTORS FROM APPLE TREE ORCHARDS IN THE CZECH REPUBLIC

R. Fialová | M. Navrátil | P. Válová | P. Lauterer

69. POMACEOUS FRUIT TREE PHYTOPLASMAS AND THEIR POTENTIAL VECTORS IN CROATIA

70. SEASONAL COLONISATION BEHAVIOUR OF CANDIDATUS PHYTOPLASMA MALI IN APPLE TREES IN TRENTINO

F. Pedrazzoli | A.M. Ciccotti | P.L. Bianchedi | A. Salvadori | R. Zorer

71. APPLE PROLIFERATION TRANSMISSION BY GRAFTING IN DIFFERENT PERIODS OF THE YEAR

72. INVESTIGATIONS WITH REAL-TIME PCR ASSAY ON THE TRANSMISSIBILITY OF PEAR DECLINE PHYTOPLASMA (PDP) WITH DORMANT BUDS

73. A REAL-TIME PCR ASSAY FOR THE DETECTION OF EUROPEAN STONE FRUIT YELLOWS PHYTOPLASMA (ESFYP) IN PLANT PROPAGATION MATERIAL

74. FIRST REPORT OF CANDIDATUS PHYTOPLASMA MALI IN GREECE AND CORRELATION WITH SMALL APPLE FRUIT DISORDER OCCURRING IN THE ORCHARDS OF THE PELION MOUNTAIN

A. Rumbou | L. Carraro | G. Nanos | I. Boutla | I.C. Rumbos

75. DETECTION OF PEAR DECLINE DISEASE IN PEAR AND QUINCE IN THE EASTERN MEDITERRANEAN REGION OF TURKEY

G. Sertkaya | E. Sertkaya | K. Kaya

76. AMELIORATION OF A REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION (RT-PCR) FOR THE DETECTION OF ASSVD, PBCVD AND PLMVD VIROIDS, AND THEIR PRESENCE IN CULTIVATED AND WILD POME AND STONE FRUITS IN GREECE

I.N. Boubourakas | C. Arambatzis | P.E. Kyriakopoulou | C. Dovas

77. PEACH LATENT MOSAIC VIROID: MAJOR FINDINGS OF OUR STUDIES OVER A PERIOD OF FIFTEEN YEARS IN ITALY

F. Faggioli | M. Barba

78. FIRST RECORD OF PEACH LATENT MOSAIC VIROID IN CROATIA

D. korić | M. Al Rwahnih | A. Myrta

79. PHENOTYPICAL VARIATION IN PEACH TREES INOCULATED WITH DEFINED MIXTURES OF VIRUSES AND PEACH LATENT MOSAIC VIROID

P.G. Gibson | G.L. Reighard | S.W. Scott | D. Marini

80. WILD HOP (HUMULUS LUPULUS L.) IS FREE OF ANY VIROID IN CAMPANIA REGION (SOUTHERN ITALY)

E. Ragozzino | F. Faggioli | A. Cirillo

81. IDENTIFICATION AND MOLECULAR CHARACTERISATION OF PEACH LATENT MOSAIC VIROID ISOLATE INFECTING PEACH IN EGYPT

M. Hassan | P. Rysanek | D. Alioto | M. Malfitano

82. DETECTION OF PEACH LATENT MOSAIC VIROID ON PEACH IN SERBIA

D. Jevremović | S. Paunović

83. DETECTION OF APPLE MOSAIC VIRUS ON APPLE CULTIVARS GROWING IN SOUTH-WEST TURKEY BY ELISA AND RT-PCR METHODS

N. Yardımcı | B. Çevik | H. Eryiğit