Articles
IN VITRO CLONAL PROPAGATION OF SEVERAL GRAPEVINE CULTIVARS
Article number
843_18
Pages
151 – 156
Language
English
Abstract
Soilless culture as in vitro culture could provide important sources of genetic variability useful for new cultivar selection.
The objective of our study was to establish the culture conditions for in vitro micropropagation of some grapevine cultivars and to identify if the somaclonal variations occur during in vitro culture.
The following cultivars were studied: Feteasca Regala, Muscat Ottonel, Brumariu and Radames. In vitro culture was induced by shoots explants.
Shoots explants regenerated plants on all media studied, but multiplication is generally low and it was obtained especially on MS media supplementd with BA and NAA. The in vitro plantlets were analysed by mean of RAPD markers in order to identify the somaclonal variations.
The best RAPD patterns have been obtained with OPE-3, OPO-4, OPO-7 and OPO-8 primers.
These markers showed differences between cultivars but the polymorphism of in vitro plantlets was not observed.
Somaclonal variation could not be identified by these RAPD markers.
In vitro multiplication was in fact a clonal propagation.
The objective of our study was to establish the culture conditions for in vitro micropropagation of some grapevine cultivars and to identify if the somaclonal variations occur during in vitro culture.
The following cultivars were studied: Feteasca Regala, Muscat Ottonel, Brumariu and Radames. In vitro culture was induced by shoots explants.
Shoots explants regenerated plants on all media studied, but multiplication is generally low and it was obtained especially on MS media supplementd with BA and NAA. The in vitro plantlets were analysed by mean of RAPD markers in order to identify the somaclonal variations.
The best RAPD patterns have been obtained with OPE-3, OPO-4, OPO-7 and OPO-8 primers.
These markers showed differences between cultivars but the polymorphism of in vitro plantlets was not observed.
Somaclonal variation could not be identified by these RAPD markers.
In vitro multiplication was in fact a clonal propagation.
Authors
A.L. Butiuc-Keul, A. Coste, B. Oltean, C. Crăciunaş, A. Halmagyi, C. Deliu, M. Farago, M. Iliescu, R. Iuoraş
Keywords
explants, shoots, micropropagation, molecular markers, RAP analysis
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