Articles
EX VITRO ACCLIMATIZATION OF LAELIA HALBINGERIANA PLANTS GROWN IN DIFFERENT MEDIA AND FERTILIZATION DOSES
Article number
843_24
Pages
191 – 196
Language
English
Abstract
In vitro plants of the orchid Laelia halbingeriana were obtained from seed germination.
The plants were grown and established in different organic substrates mixes.
The substrates resulted from the mix of compost and charcoal, land of mount and Holm oak bark, and peat and volcanic tuff.
The plants were fertilized with four levels of nitrogen (0, 10, 30 and 50 mg L-1). Six months after the establishment, 64.84% to 80.22% of the plants were adapted to the climate conditions.
The highest survival rates in plantlets were obtained in the adaptation conditions using peat-volcanic tuff and nitrogen doses of 30 and 50 mg L-1. These plants had an average of 8.01 cm height, 5.15 leafs, 2.66 pseudo-bulbs, 77 to 89 mg of total accumulated dry matter, and 42 to 45% of C in leaves and pseudo-bulbs.
The fertilization had a significant effect (P≤0.05) on the plants growth and a highly significant effect (P≤0.01) on number of leaves, width of the major leaf and the number of pseudo-bulbs; The interaction substrates and fertilization had a highly significant effect (P≤0.01) on the nitrogen content in leaves and pseudo-bulbs.
The plants were grown and established in different organic substrates mixes.
The substrates resulted from the mix of compost and charcoal, land of mount and Holm oak bark, and peat and volcanic tuff.
The plants were fertilized with four levels of nitrogen (0, 10, 30 and 50 mg L-1). Six months after the establishment, 64.84% to 80.22% of the plants were adapted to the climate conditions.
The highest survival rates in plantlets were obtained in the adaptation conditions using peat-volcanic tuff and nitrogen doses of 30 and 50 mg L-1. These plants had an average of 8.01 cm height, 5.15 leafs, 2.66 pseudo-bulbs, 77 to 89 mg of total accumulated dry matter, and 42 to 45% of C in leaves and pseudo-bulbs.
The fertilization had a significant effect (P≤0.05) on the plants growth and a highly significant effect (P≤0.01) on number of leaves, width of the major leaf and the number of pseudo-bulbs; The interaction substrates and fertilization had a highly significant effect (P≤0.01) on the nitrogen content in leaves and pseudo-bulbs.
Authors
A. Heredia-Rendón, J. Enríquez-del Valle, G.V. Campos, F. Marini, V.A. Velasco, A. Damon
Keywords
Orchidacea, in vitro, seeds, nitrogen, development
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