Articles
UTILITY OF RAPD DNA MARKERS IN THE SELECTION OF GRAFTS OF THE MEXICAN GUAVA CULTIVAR ‘MEDIA CHINA’ WITHOUT SYMPTOMS OF DEFICIENCY FOR IRON AND ZINC
Article number
849_22
Pages
203 – 210
Language
English
Abstract
This research was carried out in order to evaluate by RAPD markers (randomly amplified polymorphic DNA) the morphological and molecular variability of guava plants (Psidium guajava L.) of the cultivar Media China obtained from seeds and subjected to growth conditions of low concentrations of Fe and Zn.
The response of plants to nutrimental stress and 49 RAPD loci were associated through an analysis of grouping using the NTSYS and DARwin programs.
The analysis of RAPD patterns obtained from a sample of 82 plants showed the existence of genetic variability.
From 49 loci evaluated some 96% were polymorphic, with an index of average diversity equal to 0.744. The groups of plants without symptoms were associated to the presence of RAPD loci A20-4, A20-2, and A20-1, with a molecular weight of 0.85, 1.4 and 1.6 kb and a frequency of 74%. Due to the association of more than one locus with the characterized absence of symptoms, it is inferred that this characteristic is polygenic, the combination of the three mentioned loci being necessary to define the groups without symptoms for deficiency of Fe and Zn.
The results suggest that RAPD markers are useful to detect genetic variability and that the changes that occur at the level of the genome not always correspond to the changes in the plant phenotype.
The found variability has potential to aid in the selection of suitable genotypes as rootstocks in guava, because iron and zinc ions are the most restrictive elements in soils of alkaline pH.
The response of plants to nutrimental stress and 49 RAPD loci were associated through an analysis of grouping using the NTSYS and DARwin programs.
The analysis of RAPD patterns obtained from a sample of 82 plants showed the existence of genetic variability.
From 49 loci evaluated some 96% were polymorphic, with an index of average diversity equal to 0.744. The groups of plants without symptoms were associated to the presence of RAPD loci A20-4, A20-2, and A20-1, with a molecular weight of 0.85, 1.4 and 1.6 kb and a frequency of 74%. Due to the association of more than one locus with the characterized absence of symptoms, it is inferred that this characteristic is polygenic, the combination of the three mentioned loci being necessary to define the groups without symptoms for deficiency of Fe and Zn.
The results suggest that RAPD markers are useful to detect genetic variability and that the changes that occur at the level of the genome not always correspond to the changes in the plant phenotype.
The found variability has potential to aid in the selection of suitable genotypes as rootstocks in guava, because iron and zinc ions are the most restrictive elements in soils of alkaline pH.
Authors
J.L. Domínguez-Álvarez, G. Aguilar-Sanchez, D. Nieto-Angel, J.P. Legaria-Solano, J. Pineda-Pineda
Keywords
genetic variability, fertilization, statistical analysis
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