IDENTIFICATION OF PROGENIES DERIVED FROM SEXUAL HYBRIDIZATION OF CITRUS USING RAPD MOLECULAR MARKER

In this study, the RAPD (random amplified polymorphic DNA) technology was used to identify citrus progenies from six sexual hybrid combinations.
Firstly, a suitable RAPD reaction system for citrus was established, i.e. 1.2-fold 10×buffer, 0.2 mM dNTP, 0.2 μM optimum primers, 1.2 U Taq DNA polymerase and 0.8 ng/μl DNA templates in a 25 μl reaction volume.
NTsys software was used for data analysis and respective dendrogram depicted by Jaccard’s similarity coefficients using UPGMA method.
Six hundred seventy-one F1 individuals from six different hybrid combinations were tested in their early stages of development and 647 individuals were identified as true hybrids.
The similarity coefficients for maternal and paternal materials ranged from 0.655 to 0.836. The highest similarity coefficient (0.836) was observed between ‘Kiyomi’ and ‘Chuntianju’ compared to the lowest between ‘Kiyomi’ and ‘Shatian Polemo’. The results indicated that RAPD technology was applicable to the identification of citrus hybrid sexual progenies in their early stages of development.

I International Symposium on Tropical Horticulture

Y.H. Qin, H.G. Hu, Z.X. Ye, S.Q. Lin, H.X. Miao, C.Y. Zhang, G.B. Hu

citrus, hybridization, RAPD, identification, genetic relationship

https://doi.org/10.17660/ActaHortic.2011.894.12