Articles
STANDARDIZED PROTOCOL FOR EGGPLANT SEED REGENERATION AND SEED STORAGE AT AVRDC – THE WORLD VEGETABLE CENTER
Article number
898_9
Pages
81 – 87
Language
English
Abstract
Eggplant (Solanum melongena L.), a vegetable popular around the world, likely originated from South and Southeast Asia, where wide genetic diversity exists.
It is one of the principal crops of AVRDC The World Vegetable Centers genebank, which maintains a collection of 3390 accessions at its Chinese Taipei headquarters for plant breeders and other users worldwide.
To preserve the original traits of the germ¬plasm during seed regeneration, processing, and storage, a standardized regeneration protocol has been developed and is strictly followed.
This paper gives a detailed account of the eggplant seed regeneration protocol under field conditions.
To minimize the risk of Tobacco mosaic virus (TMV) infection during the regeneration cycle, dry seeds are treated with trisodium phosphate before sowing in seedling trays in the screenhouse.
Thinning to one seedling per cell is done when seedlings have one to two true leaves.
About five weeks after sowing, seedlings are transplanted inside net cages in the field to prevent insect damage and accidental outcrossing.
Six eggplant accessions can be grown in one net cage of 112.5 m2 (22.5×5 m) in double-row plots.
The plot size is 9 m2 (6×1.5 m), consisting of 30 plants with a spacing of 40×75 cm.
Eggplant is allowed to self-pollinate.
Supplementary hand pollination is done to enhance seed yield.
Disease and pest management focuses on grey mold rot, thrips, leaf hopper, spider mite, aphids, and the eggplant fruit and shoot borer.
Harvested fruit is kept for one week at room temperature before extracting seed by hand.
Seed is dried on seed screens in a drying room at 15°C and 15% RH for 7-10 days to adjust the seed moisture content to 5-6% before processing and storage.
It is one of the principal crops of AVRDC The World Vegetable Centers genebank, which maintains a collection of 3390 accessions at its Chinese Taipei headquarters for plant breeders and other users worldwide.
To preserve the original traits of the germ¬plasm during seed regeneration, processing, and storage, a standardized regeneration protocol has been developed and is strictly followed.
This paper gives a detailed account of the eggplant seed regeneration protocol under field conditions.
To minimize the risk of Tobacco mosaic virus (TMV) infection during the regeneration cycle, dry seeds are treated with trisodium phosphate before sowing in seedling trays in the screenhouse.
Thinning to one seedling per cell is done when seedlings have one to two true leaves.
About five weeks after sowing, seedlings are transplanted inside net cages in the field to prevent insect damage and accidental outcrossing.
Six eggplant accessions can be grown in one net cage of 112.5 m2 (22.5×5 m) in double-row plots.
The plot size is 9 m2 (6×1.5 m), consisting of 30 plants with a spacing of 40×75 cm.
Eggplant is allowed to self-pollinate.
Supplementary hand pollination is done to enhance seed yield.
Disease and pest management focuses on grey mold rot, thrips, leaf hopper, spider mite, aphids, and the eggplant fruit and shoot borer.
Harvested fruit is kept for one week at room temperature before extracting seed by hand.
Seed is dried on seed screens in a drying room at 15°C and 15% RH for 7-10 days to adjust the seed moisture content to 5-6% before processing and storage.
Publication
Authors
A.W. Ebert , Tien-hor Wu
Keywords
Solanum melongena, seed treatment, seed drying, virus infections, net cages, hand pollination, disease and pest management
Online Articles (41)