Articles
F1 HYBRID PINEAPPLE RESISTANT TO BIALAPHOS HERBICIDE
Article number
902_20
Pages
201 – 207
Language
English
Abstract
Weeds are the main cost factor for pineapple production as at least a half of production costs are related to weed control.
All current commercial pineapple cultivars are sensitive or susceptible to biodegradable herbicides.
This research project aimed to establish an F1 hybrid pineapple cultivar resistant to herbicide by conventional breeding.
Direct and reciprocal crosses between genetically modified Phuket pineapple (TP) carrying the bialaphos-resistant (bar) gene and the commercial pineapple cultivars Pattavia (PV) and Phuket (PK) were made in 2005 at the Faculty of Agriculture, Rajamangala University of Technology Srivijaya, Nakhonsithammarat, Thailand.
The crosses resulted in 1,172 F1 seeds and 886 F1 seedlings were obtained from the first crossing of the two crosses.
No seeds were obtained from selfing of TP, which shows that transforming Phuket did not eliminate the incompatibility of TP×TP. The cross TP and PK also did not produce seeds but 14 seeds were obtained from the reciprocal cross of PK×TP showing that transformed Phuket was at least somewhat compatible with the untransformed Phuket. The 886 F1 plants were evaluated for agronomic characteristics, fruit quality and bialaphos herbicide resistance.
There were 410 F1 plants that showed resistance to bialaphos herbicide.
Furthermore, there were 18 F1 plants showing other good agronomic characters and good fruit quality.
Three promising hybrids, (PV×TP)-51; (PV×TP)-46 and (PV×TP-34), showed resistant to biodegradation bialaphos herbicide, a °Brix of 18-22.2, a fragrant smell, crispy flesh and a smooth leaf with only the tip being spiny.
In particular, (PV×TP)-34 hybrid plants had pale-pink bracts and fruit with a 22.2°Brix, cylindrical fruit shape, dark yellow flesh, and pleasant flavor.
These potential hybrids will be propagated and evaluated to confirm the traits and subsequently released to farmers.
All current commercial pineapple cultivars are sensitive or susceptible to biodegradable herbicides.
This research project aimed to establish an F1 hybrid pineapple cultivar resistant to herbicide by conventional breeding.
Direct and reciprocal crosses between genetically modified Phuket pineapple (TP) carrying the bialaphos-resistant (bar) gene and the commercial pineapple cultivars Pattavia (PV) and Phuket (PK) were made in 2005 at the Faculty of Agriculture, Rajamangala University of Technology Srivijaya, Nakhonsithammarat, Thailand.
The crosses resulted in 1,172 F1 seeds and 886 F1 seedlings were obtained from the first crossing of the two crosses.
No seeds were obtained from selfing of TP, which shows that transforming Phuket did not eliminate the incompatibility of TP×TP. The cross TP and PK also did not produce seeds but 14 seeds were obtained from the reciprocal cross of PK×TP showing that transformed Phuket was at least somewhat compatible with the untransformed Phuket. The 886 F1 plants were evaluated for agronomic characteristics, fruit quality and bialaphos herbicide resistance.
There were 410 F1 plants that showed resistance to bialaphos herbicide.
Furthermore, there were 18 F1 plants showing other good agronomic characters and good fruit quality.
Three promising hybrids, (PV×TP)-51; (PV×TP)-46 and (PV×TP-34), showed resistant to biodegradation bialaphos herbicide, a °Brix of 18-22.2, a fragrant smell, crispy flesh and a smooth leaf with only the tip being spiny.
In particular, (PV×TP)-34 hybrid plants had pale-pink bracts and fruit with a 22.2°Brix, cylindrical fruit shape, dark yellow flesh, and pleasant flavor.
These potential hybrids will be propagated and evaluated to confirm the traits and subsequently released to farmers.
Publication
Authors
S. Sripaoraya, M.R. Davey, P. Srinives
Keywords
Ananas comosus var. comosus, bar gene, crossing, glufosinate, weed, biodegradation
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