Articles
MICROARRAY ANALYSIS OF BLUE OR YELLOW WEAK LIGHT EFFECT TO GENES IN ARABIDOPSIS
Article number
907_60
Pages
367 – 370
Language
English
Abstract
Plants morphologically respond to light quality in a number of physiological ways.
At the cDNA level, the expression of some genes changes by the light quality.
Very weak light quality responses to plants have important information for crop production techniques with supplemental lighting in dark period.
To get fundamental knowledge this time, novel genes of Arabidopsis that genetic information is almost elucidated were identified regulated in this way.
We used microarrays containing 21,500 Arabidopsis expressed sequence tags, representing an estimated 16,337 unique genes, to determine gene expression levels under very different light qualities, blue, yellow and white as control light, with very weak intensity less than 2 µmol m-2 s-1, using a band pass filter and fluorescent lamps. 76% of the genes, encompassing genes expressed at both high and low levels, showed a different expression pattern.
Approximately 42 and 34% of the genes was increased with yellow light and blue light, respectively. 498 genes were increased more than double with yellow light in comparison with white light.
On the other hand, 652 genes were increased more than double with blue light in comparison with white light.
Furthermore, 23 genes for which quantity of expression of the yellow light came across more than 3 times in comparison with white light, and 61 genes for which quantity of expression of the blue light came across more than 3 times in comparison with white light.
Groups of genes regulated by photosynthesis, circadian rhythm, response to absence of light, etc. were identified.
At the cDNA level, the expression of some genes changes by the light quality.
Very weak light quality responses to plants have important information for crop production techniques with supplemental lighting in dark period.
To get fundamental knowledge this time, novel genes of Arabidopsis that genetic information is almost elucidated were identified regulated in this way.
We used microarrays containing 21,500 Arabidopsis expressed sequence tags, representing an estimated 16,337 unique genes, to determine gene expression levels under very different light qualities, blue, yellow and white as control light, with very weak intensity less than 2 µmol m-2 s-1, using a band pass filter and fluorescent lamps. 76% of the genes, encompassing genes expressed at both high and low levels, showed a different expression pattern.
Approximately 42 and 34% of the genes was increased with yellow light and blue light, respectively. 498 genes were increased more than double with yellow light in comparison with white light.
On the other hand, 652 genes were increased more than double with blue light in comparison with white light.
Furthermore, 23 genes for which quantity of expression of the yellow light came across more than 3 times in comparison with white light, and 61 genes for which quantity of expression of the blue light came across more than 3 times in comparison with white light.
Groups of genes regulated by photosynthesis, circadian rhythm, response to absence of light, etc. were identified.
Authors
L. Okushima, S. Sase, Y. Murata, Y. Iwahashi, N. Fukuda
Keywords
bandpass filter, dark period, DNA microarray, supplemental lightning
Online Articles (68)