Articles
CDNA MICROARRAY ANALYSIS OF DIFFERENTIAL GENE EXPRESSION IN TOMATO FRUITS EXPOSED TO BLUE, UV-A, AND UV-A+UV-B
Article number
907_61
Pages
371 – 374
Language
English
Abstract
Plants of tomato Micro Tom were grown under metal-halide lamps with a UV-safe acrylic plate installed between the lights and plants.
Plants with fruits at the breaker stage were transferred to a growth chamber equipped with white fluorescent lamps filtered with UV-A-absorbing (<370 nm) acrylic plates.
Four hours after the start of the light period, the fruit were irradiated with one of three types of supplemental light sources: blue LED, UV-A fluorescent lamps (UV-A+UV-B), and UV-A fluorescent lamps filtered through a UV-B-safe glass plate (UV-A). Microarray hybridization was performed on all combinations of cDNAs from blue, UV-A, UV-A+UV-B, and white light groups.
The gene expression profile of the UV-A group was similar to that of the control (white light), and a few genes were up-regulated slightly by blue light.
In contrast, the expression of several genes was down-regulated by UV-A+UV-B. Many of these genes were categorized as those involved in transcriptional regulation and signal transduction.
Plants with fruits at the breaker stage were transferred to a growth chamber equipped with white fluorescent lamps filtered with UV-A-absorbing (<370 nm) acrylic plates.
Four hours after the start of the light period, the fruit were irradiated with one of three types of supplemental light sources: blue LED, UV-A fluorescent lamps (UV-A+UV-B), and UV-A fluorescent lamps filtered through a UV-B-safe glass plate (UV-A). Microarray hybridization was performed on all combinations of cDNAs from blue, UV-A, UV-A+UV-B, and white light groups.
The gene expression profile of the UV-A group was similar to that of the control (white light), and a few genes were up-regulated slightly by blue light.
In contrast, the expression of several genes was down-regulated by UV-A+UV-B. Many of these genes were categorized as those involved in transcriptional regulation and signal transduction.
Authors
S. Kawabata, K. Miyamoto, Y. Li
Keywords
ultraviolet-A, ultraviolet-B, flavonoid, carotenoid, Solanum lycopersicum, breaker stage
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