Home > XI International Pear Symposium > PEAR FRUIT TASTE MODIFICATION BY THAUMATIN II GENE EXPRESSION

Articles

PEAR FRUIT TASTE MODIFICATION BY THAUMATIN II GENE EXPRESSION

Article number

909_5

Pages

67 – 73

Language

English

Abstract

The gene for supersweet protein thaumatin II from Thaumatococcus danielli has been transferred to pear in attempts to improve fruit taste and phytopathogen resistance.
Based on the coding sequence, cloned at Unilever, and plasmid pBI121, we constructed the binary vector for its expression in plants.
The successful transformation of the pear cultivar Burakovka has been obtained by using the disarmed supervirulent agrobacterial strain CBE21. The introduction of thaumatin gene was confirmed by PCR analysis and its expression in different tissues by western blotting.
Fruit from the transgenic line demonstrated taste modification, detectable by organoleptic test.

Publication

XI International Pear Symposium

Authors

S.V. Dolgov, V.G. Lebedev, F.P. Firsov

Keywords

Pyrus communis, agrobacterial transformation, thaumatin, taste modification

Full text

https://doi.org/10.17660/ActaHortic.2011.909.5

Online Articles (95)

1. ECONOMIC EVALUATION BETWEEN CHEMICAL THINNING VS. HAND THINNING IN ‘WILLIAMS’ PEAR

2. REGIONAL ECONOMIC BALANCE OF THE PEAR INDUSTRY IN THE UPPER RÍO NEGRO AND NEUQUÉN VALLEY

H. Zubeldía | M. Leskovar

3. STRATEGIES FOR FIRE BLIGHT RESISTANCE BREEDING IN PEAR (PYRUS COMMUNIS); 30 YEARS OF EXPERIENCE

Y. Lespinasse | P. Guérif | C.-E. Durel

4. IS DWARFING IN PEAR ROOTSTOCKS DUE TO ANEUPLOID GENETIC STRUCTURES?

5. PEAR FRUIT TASTE MODIFICATION BY THAUMATIN II GENE EXPRESSION

S.V. Dolgov | V.G. Lebedev | F.P. Firsov

6. QUINCE (CYDONIA OBLONGA) GENETIC RELATIONSHIPS DETERMINED USING MICROSATELLITE MARKERS

7. CHILLING REQUIREMENT FOR DORMANCY BUD BREAK IN EUROPEAN PEAR

8. THE HUNGARIAN PEAR GERMPLASM (PYRUS COMMUNIS) AS SOURCE OF GENETIC VARIABILITY FOR BREEDING PROGRAMS

I. Labuschagne | S. Musacchi | J. Nyéki | Z. Szabó | T. Szabó

9. INHERITANCE OF RED LEAF COLOUR FROM PEAR RED SPORTS OF ‘DOYENNÉ DU COMICE’, ‘BARTLETT’ AND ‘BEURRÉ HARDY’

Y. Lespinasse | P. Guérif

10. GLOBAL PEAR BREEDING PROGRAMMES: GOALS, TRENDS AND PROGRESS FOR NEW CULTIVARS AND NEW ROOTSTOCKS

L.R. Brewer | J.W. Palmer

11. THE IRTA-PFR PEAR SCION BREEDING PROGRAMME FOR HIGH FRUIT QUALITY

12. HERITABILITY AND PARENTAL BREEDING VALUE ESTIMATES OF ABRASION-INDUCED SKIN DISCOLOURATION ON PEAR FRUIT

L.R. Brewer | C.G.T. Morgan | P.A. Alspach | R.K. Volz

13. CHARACTERIZATION OF COLD HARDINESS IN QUINCE: POTENTIAL PEAR ROOTSTOCK CANDIDATES FOR NORTHERN PEAR PRODUCTION REGIONS

T.C. Einhorn | J. Turner | D. Gibeaut | J.D. Postman

14. THE BRAZILIAN PEAR BREEDING PROGRAM

15. EVALUATION OF PEAR ROOTSTOCK SELECTIONS

16. THE PEAR INDUSTRY AND RESEARCH IN CHINA

Yuanwen Teng

17. CULTIVAR TESTING OF PEARS IN BELGIUM

J. Vercammen | A. Gomand | H. Goossens

18. PERFORMANCE OF ‘US 71655-014’, A FIRE BLIGHT RESISTANT PEAR SELECTION FROM THE USA

S. Castagnoli | J. Turner | T.C. Einhorn | R. Bell

19. EVALUATION OF POTENTIAL ROOTSTOCKS TO IMPROVE PEAR TREE PRECOCITY AND PRODUCTIVITY

20. AGRONOMICAL PERFORMANCE AND FRUIT QUALITY OF ‘CONFERENCE’ ON SOME CYDONIA AND PYRUS ROOTSTOCKS

I. Iglesias | I. Batlle

21. EVALUATION OF REGIONAL CULTIVARS IN A PEAR GENE BANK IN HUNGARY

G.M. Kocsisne | L. Kocsis | Z. Szabó | J. Nyéki | M. Soltész

22. VEGETATIVE ASPECTS OF EUROPEAN PEAR SCIONS CULTIVARS IN COMBINATION WITH QUINCE ROOTSTOCKS IN URUPEMA SANTA CATARINA STATE, BRAZIL

23. PERFORMANCE OF ‘COMICE’ PEAR ON QUINCE ROOTSTOCKS IN OREGON, USA

D. Sugar | S.R. Basile

24. EVALUATION OF THE GROWTH AND RESPONSES OF IN VITRO CULTURES OF SEVERAL PEAR ROOTSTOCKS EXPOSED TO SIMULATED DROUGHT STRESS USING POLYETHYLENE GLYCOL TREATMENTS

25. TRAINING SYSTEM AND MANAGEMENT FOR A HIGH DENSITY ORCHARD OF ‘ABBÉ FETEL’

S. Musacchi

26. COMPARISON OF PLATFORM VERSUS LADDERS FOR HARVEST IN NORTHERN CALIFORNIA PEAR ORCHARD

27. COMPARISON AMONG PEAR TRAINING SYSTEMS AND ROOTSTOCKS FOR HIGH DENSITY PLANTING (HDP) OF THE CULTIVAR ‘ABBÉ FÉTEL’

S. Musacchi | S. Serra | V. Ancarani

28. HIGH DENSITY PEAR PRODUCTION WITH PYRUS COMMUNIS ROOTSTOCKS

T.L. Robinson

29. COMPARISON AMONG DIFFERENT PLANTING SYSTEMS FOR ‘CONFERENCE’

J. Vercammen

30. TRAINING SYSTEMS AND ROOTSTOCKS FOR HIGH DENSITY PLANTING (HDP) OF THE CULTIVAR ‘ABBÉ FÉTEL’: DEVELOPMENTAL TRIALS IN ITALY

F. Galli | V. Ancarani | S. Serra | S. Musacchi

31. EFFECT OF IRRIGATION AND NITROGEN OR POTASSIUM FERTIGATION ON YIELD AND FRUIT QUALITY ON ‘CONFERENCE’ PEAR TREES IN BELGIUM

T. Deckers | W. Verjans | H. Schoofs | P. Janssens | F. Elsen

32. RESPONSE OF ‘LE-CONTE’ PEAR TREES TO SOIL BORON APPLICATION

S.M. El-Shazly | H.R. Kotb

33. PHYSICAL AND PHYSICO-CHEMICAL PROPERTIES OF SALINE SOILS AND EFFECTS ON YIELD AND QUALITY OF ‘WILLIAMS’ PEAR IN THE UPPER RIO NEGRO VALLEY, ARGENTINA

M.C. Aruani | N. Barnes | G. Striebeck | B. Osre | Y. Machuca

34. NEMATODE COMMUNITY STRUCTURE AND CHEMICAL AND PHYSICAL PROPERTIES IN DIFFERENT TEXTURAL CLASSES OF SOIL IN THE ROOT ZONE OF ‘WILLIAMS’ PEAR

C.V. Azpilicueta | M.C. Aruani | L. Vitta

35. DIFFERENTIAL NITROGEN UPTAKE DURING GROWTH CYCLE IN YOUNG ‘ROCHA’ PEAR TREES

M. Mota | C.B. Neto | C.M. Oliveira

36. EFFECT OF GROUNDCOVER ON AVAILABLE MACRONUTRIENT CONTENT OF SOIL AND PLANT UPTAKE IN NON-BEARING PEAR ORCHARD

T. Nagy | J. Nyéki | Z. Szabó | G.M. Kocsisne

37. WATER POTENTIAL AND FRUIT GROWTH AS MANAGEMENT INDICATORS IN TRICKLE-IRRIGATION IN ‘ROCHA’ PEAR

A. Pacheco | J. Azevedo | C.M. Oliveira

38. WATER USE BY DRIP IRRIGATED ONE-YEAR OLD PEAR TREES

A. Requena | G. Nordenströn | E. Castillo

39. EFFECTIVENESS OF AQUEOUS VEGETAL EXTRACTS IN NUTRITION MANAGEMENT OF PEAR TREES

G. Sorrenti | E. Baldi | M. Toselli | B. Marangoni

40. SUSTAINABLE USE OF PLANT BIOREGULATORS IN PEAR PRODUCTION

M.C. Dussi

41. CROP LOAD REGULATION IN ‘CONFERENCE’ PEARS

F.M. Maas | P.A.H. van der Steeg

42. FRUIT SET CONTROL IN PEAR CULTIVARS ‘ABATE FETEL’ AND ‘PACKHAM’S TRIUMPH’

43. EVALUATION OF 6-BENZYLADENINE (BA) AND NAPHTHYLACETAMIDE (NAD) AS POST-BLOOM THINNING COMPOUNDS FOR ‘EARLY BON CHRÉTIEN’ PEAR

K.I. Theron | T.G. Chabikwa | G.F.A. Lötze

44. EFFECT OF CONCENTRATION, APPLICATION VOLUME AND ADDITION OF A SURFACTANT ON RESPONSE TO BENZYLADENINE AS THINNING AGENT IN ‘WILLIAMS’ PEARS

M. Curetti | R. Rodríguez | C. Magdalena | A. Rodríguez

45. FRUIT THINNING AND FRUIT SIZE ENHANCEMENT WITH 6-BENZYLADENINE APPLICATION TO ‘WILLIAMS’ PEAR

M.C. Dussi | D. Sugar

46. NECTAR PRODUCTION AND QUALITY IN JAPANESE PEAR CULTIVARS IN SOUTH BRAZIL

I.D. Faoro | A.I. Orth

47. PARTHENOCARPY IN JAPANESE PEAR TREE CULTIVARS IN SOUTH BRAZIL

I.D. Faoro | A.I. Orth

48. EVALUATION OF FIVE DORMANCY BREAKING AGENTS TO INDUCE SYNCHRONIZED FLOWERING IN ‘ROCHA’ PEAR

N. Pereira | C.M. Oliveira | M. Mota | R.M. Sousa

49. PLANT GROWTH REGULATORS INCREASE PRODUCTIVITY OF ‘PACKHAM’S TRIUMPH’ PEAR IN SOUTHERN BRAZIL

50. EFFECT OF AVG APPLICATION ON FRUIT SET, YIELD AND FRUIT SIZE IN ‘ABATE FETEL’ AND ‘PACKAM’S TRIUMPH’ PEARS IN A SEMI-COMMERCIAL STATISTICAL TRIAL

E. Sánchez | M. Curetti | J. Retamales

51. THE EFFECT OF MAXIM^® (3, 5, 6-TPA) IN PREVENTING PRE-HARVEST FRUIT DROP IN ‘CONFERENCE’ AND ‘BLANQUILLA’ PEAR

E. Torres | L. Asín | S. Alegre

52. TRENDS IN CODLING MOTH MATING DISRUPTION PROGRAMS IN THE PEAR ORCHARDS OF ARGENTINA

L. Cichón

53. EUROPEAN PEAR CULTIVAR RESISTANCE TO BIO-PESTS: SCAB (VENTURIA PIRINA) AND PSYLLA (CACOPSYLLA PYRI)

54. MICROSCOPIC STUDIES OF SUSCEPTIBILITY AND RESISTANCE SYMPTOMS EXPRESSED ON TWO CULTIVARS OF PYRUS COMMUNIS INOCULATED WITH VENTURIA PIRINA

M. Chevalier | M. Tellier | Y. Lespinasse | R. Mallet

55. EVALUATION OF PHOSPHINE GAS AS A QUARANTINE TREATMENT FOR OBSCURE MEALYBUG FOR EXPORT MARKETS

56. EPIDEMIOLOGY AND EFFECTIVE CONTROL OF ALTERNARIA ALTERNATE, CAUSAL AGENT OF DEAD (DORMANT) FLOWER BUD DISEASE OF PEAR

M. Wenneker | N. Joosten | R. Anbergen | P. Vink | A.S. van Bruggen

57. CURRENT OCCURRENCE OF THE MAJOR LEPIDOPTERA PESTS IN THE ORIENTAL PEAR ORCHARDS IN KOREA

Young Sik Cho | J.H. Song | H.S. Hwang | Hyunwook Jo | Chon-Woo Park

58. OBSCURE MEALYBUG (PSEUDOCOCCUS VIBURNI) IN PEAR TREES IN THE ALTO VALLE OF RIO NEGRO AND NEUQUEN, ARGENTINA

G.L. Dapoto | A. Olave | M. Bondoni | H. Giganti

59. FRUIT ROT CAUSED BY PHYTOPHTHORA SP. IN COLD-STORED PEARS IN THE VALLEY OF RIO NEGRO AND NEUQUÉN, ARGENTINA

60. TREATMENTS WITH NON-PHYTOTOXIC CONCENTRATIONS OF CU AND MN AND THE INDUCTION OF DEFENCE MECHANISMS IN PEAR TREES AGAINST INFECTION WITH ERWINIA AMYLOVORA

K. Ghesquiere | K. Vrancken | R. Valcke | H. Schoofs | T. Deckers

61. PREDICTION OF SYMPTOMS OCCURRENCE OF FIRE BLIGHT (ERWINIA AMYLOVORA) BY THE MARYBLYT^TM PROGRAM IN RÍO NEGRO, ARGENTINA

A. Giayetto | M. Rossini

62. DETECTION OF PHYTOPHTHORA CACTORUM IN THE IRRIGATION WATER IN COMMERCIAL ORCHARDS OF ‘BARTLETT’ PEAR IN VILLA REGINA, RÍO NEGRO, ARGENTINA

V.I. Rivero | A. Giayetto | M. Rossini | D. Vera

63. POTENTIAL ORGANIC METHODS FOR MANAGEMENT OF PEAR SCAB

D. Sugar | R.J. Hilton

64. EVOLUTION OF SECONDARY PESTS IN CALIFORNIA PEAR ORCHARDS UNDER MATING DISRUPTION FOR CODLING MOTH CONTROL

L.G. Varela | R.B. Elkins | R.A. Van Steenwyk

65. SIZE MATTERS: FACTORS INFLUENCING FRUIT SIZE IN PEAR

K.I. Theron

66. ORCHARD COOLING WITH OVERTREE MICROSPRINKLER IRRIGATION TO INCREASE FRUIT RUSSET ON ‘CONFERENCE’ PEAR

L. Asín | E. Torres | P. Vilardell

67. REFLECTIVE FABRIC IMPROVES INTRA-CANOPY LIGHT LEVELS AND INCREASES YIELD IN A LOW-DENSITY, MATURE ‘D’ANJOU’ PEAR ORCHARD

T.C. Einhorn | J. Turner | D. Laraway

68. EVALUATION OF WEED AND NUTRIENT MANAGEMENT PRACTICES IN ORGANIC PEAR ORCHARDS

69. PHYSICAL, SENSORY AND CONSUMER ANALYSIS OF EATING QUALITY AND APPEARANCE OF PEAR GENOTYPES AMONG SOUTH AFRICAN CONSUMERS

W.J. Steyn | N. Manning | M. Muller | J.P. Human

70. NUTRITIONAL AND COMPOSITIONAL CHARACTERIZATION OF ARGENTINEAN ‘WILLIAMS BON CHRETIEN’ PEARS

N. Barda

71. SENSORY EVALUATION OF PRINCIPAL PEAR CULTIVARS FROM NORTH PATAGONIA, ARGENTINA

P. Calvo | C. Gittins | M.J. Miranda | N. Barda

72. EFFECT OF SEAWEED EXTRACT ON PEAR FRUIT QUALITY AND YIELD

G.M. Colavita | N. Spera | V. Blackhall | G.M. Sepulveda

73. EFFECT OF KAOLIN PARTICLE FILMS ON THE TEMPERATURE AND SOLAR INJURY OF PEAR FRUITS

G.M. Colavita | V. Blackhall | S. Valdez

74. DEVELOPING A SENSORY DESCRIPTIVE PANEL FOR PEAR QUALITY EVALUATION

C. Gittins | P. Calvo | M.J. Miranda | N. Barda

75. BAGGING OF ‘CONCORDE’ PEARS (PYRUS COMMUNIS L.) INFLUENCES FRUIT QUALITY

M. Hudina | F. Stampar

76. MICROCLIMATE MODIFICATION IN PEAR PLANTATION BY USING COOLING IRRIGATION

77. EFFECTS OF VOLUME RATE AND SPRAY PRESSURE OF AN AIR-ASSISTED SPRAYER ON PESTICIDE DISTRIBUTION AND DRIFT ON PEAR PREES

78. EFFECTS OF TRUNK GIRDLING ON FRUIT PRODUCTION, FRUIT SIZE AND TREE VIGOR ON ‘BARTLETT’ PEARS IN RÍO NEGRO AND NEUQUÉN VALLEY, ARGENTINA

79. SENSORY EVALUATION OF PEARS: A USEFUL TOOL TO DETECT CHANGES IN EATING QUALITY DURING RIPENING

80. CONTRIBUTIONS OF EARLY SEASON TEMPERATURES TO PYRUS COMMUNIS ‘BARTLETT’ FRUIT GROWTH

A. Rodríguez | E. Sánchez | A. de la Casa

81. FRUIT GROWTH AND CELL EVOLUTION IN ‘WILLIAMS’ PEARS

K. Zon | G.M. Sepúlveda | G. Giménez | M.C. Dussi

82. ADVANCES IN POSTHARVEST MANAGEMENT OF PEARS

D. Sugar

83. TEMPERATURE AND ETHYLENE: TWO USEFUL TOOLS TO BE USED IN COMBINATION WITH SMARTFRESH^SM (1-MCP) FOR DELIVERING OPTIMAL QUALITY PEARS

A. Cucchi | G. Regiroli

84. FACTORS AFFECTING SENSITIVITY OF ‘ABATE FETEL’ PEARS TO FRICTION DISCOLORATION

T. Gomila | G. Calvo | A.P. Candan

85. DEVELOPMENT OF A PRE-HARVEST PREDICTION MODEL FOR RIPENING OF ‘FORELLE’ PEARS (PYRUS COMMUNIS L.)

E. Lötze | A. Sadie | K.I. Theron

86. STRATEGIES FOR APPLYING 1-METHYLCYCLOPROPENE (1-MCP) TO ‘PACKHAM’S TRIUMPH’ PEARS

87. STORAGE OF PEARS USING DYNAMIC CONTROLLED-ATMOSPHERE (DCA), A NON-CHEMICAL METHOD

R.K. Prange | J.M. DeLong | A.H. Wright

88. HARVEST MATURITY, CONDITIONING TEMPERATURE, AND ETHYLENE TREATMENT INFLUENCE INDUCTION OF RIPENING CAPACITY IN ‘D’ANJOU’ PEAR FRUIT

D. Sugar | T.C. Einhorn

89. POST-HARVEST PERFORMANCE OF ‘ABATE FETEL’ PEARS GROWN IN ARGENTINA IN RELATION TO HARVEST TIME

G. Calvo | A.P. Candan | T. Gomila

90. RIPENING INDUCTION OF ‘PACKHAM’S TRIUMPH’ PEARS TREATED WITH 1-METHYLCYCLOPROPENE (1-MCP)

A.P. Candan | G. Calvo

91. USE OF 1-METHYLCYCLOPROPENE (1-MCP) AS A STRATEGY TO IMPROVE POST-HARVEST LIFE OF ‘ABATE FETEL’ PEARS

B.G. Defilippi | D. Manríquez | P. Robledo

92. RELATIONSHIP BETWEEN MATURITY INDEX AND ETHYLENE PRODUCTION PATTERNS OF ‘WILLIAMS’ PEARS GROWN IN THE ALTO VALLE OF RIO NEGRO

T. Gomila | G. Calvo | A.P. Candan

93. NON-DESTRUCTIVE INDEX TO CHARACTERIZE THE MATURITY OF ‘WILLIAMS’ PEARS GROWN IN ARGENTINA

T. Gomila | G. Calvo | A.P. Candan

94. EFFECT OF DIFFERENT CA STORAGE CONDITIONS ON STORABILITY AND FRUIT QUALITY OF ORGANICALLY GROWN ‘UTA’ PEARS

G. Lafer

95. TWO SELECTION STRATEGIES OF EPIPHYTIC NATIVE YEASTS WITH POTENTIAL BIOCONTROL CAPACITY AGAINST POSTHARVEST PEAR PATHOGENS IN PATAGONIA

M.C. Lutz | A. Robiglio | M.C. Sosa | C.A. Lopes | M.P. Sangorrín