ESTABLISHMENT OF EFFICIENT CALLUS INDUCTION SYSTEM OF ASTRAGALUS MONGHOLICUS BGE.

Astragali radix, the dry root of Astragalus mongholicus Bge., has been used for centuries as an important medicine to reinforce vital energy, and to promote the discharge of pus and the growth of new tissue.
Since Astragalus mongholicus Bge. has a long cultivation cycle and is greatly impacted by the environment production can be unstable and growers can hardly satisfy the market demand.
The establishment of suspension cell lines is the best solution to address this problem.
This study uses MS medium to study the different concentrations and ratios of hormones, different explants and light on the impact of the formation of callus of Astragalus mongholicus Bge.
The results showed that in MS medium with 1.0 mg/L 2,4-D added the rate of callus induction was 50.00%. 2,4-D had a much better effect on induction than 1.0 mg/L of NAA (the rate was 26.32%), and callus grown was in good condition.
The best combination of imposed concentration was 2.0 mg/L 2,4-D +1.0 mg/L 6-BA, the rate of callus induction was as high as 93.33%, and time to callus growth callus was as early as 2~3 days.
The order from high to low of rates of induction of different explants was cotyledons, hypocotyl and radicle, but cotyledons’ growth status was not as good as hypocotyl growth status.
Compared with the dark culture, light culture was more conducive to callus induction and growth.
The rates of hypocotyls, cotyledons and radicle induction were 83.33, 93.33 and 46.67%, while for the dark the rates were 73.33, 90.0 and 26.67%, respectively.
This study will provide the foundation of establishment of suspension cell lines of Astragalus mongholicus Bge.

XXVIII International Horticultural Congress on Science and Horticulture for People (IHC2010): A New Look at Medicinal and Aromatic Plants Seminar

Liu YaJing, Xing Juzhan , Chen Guilin

Astragalus mongholicus, callus induction, 2,4-D,6-BA

https://doi.org/10.17660/ActaHortic.2011.925.39