Articles
POLYPLOIDY INDUCTION OF THREE LILIUM SPECIES ENDEMIC TO CHINA (LILIUM PUMILUM, L. SARGENTIAE, L. TSINGTAUENSE)
Article number
935_11
Pages
83 – 90
Language
English
Abstract
This study was conducted to obtain tetraploids of lily plants in three Lilium species (L. pumilum, L. sargentiae, L. tsingtauense) by treating with colchicine (0, 0.02, and 0.04%) and oryzalin (0, 0.006, and 0.01%) for 24 and 48 hours.
In colchicine treatments, the highest percentage of tetraploids was obtained for the 24 hour treatment with 0.04% colchicine for L. pumilum. Tetraploids were obtained by treating with colchicine at 0.02 and 0.04% for 48 hours for L. sargentiae and L. tsingtauense. The optimal oryzalin concentration for the induction of tetraploids was 0.01% for L. tsingtauense and L. sargentiae with 24 or 48 hours treatment, while 0.01% with 24 hours and 0.006% with 48 hours were the optimal oryzalin treatment for L. pumilum. Oryzalin was more efficient than colchicine in inducing tetraploids in the lily species investigated and may be considered as an alternative reagent of colchicine.
Duration of treatment and concentration of reagent both had significant effects on the survival rate, bulblet formation, and chromosome doubling.
Morphological and anatomical characteristics of leaves and stomatal characteristics correlated with ploidy of lily plants.
Chromosome counts and flow cytometry confirmed these findings.
In colchicine treatments, the highest percentage of tetraploids was obtained for the 24 hour treatment with 0.04% colchicine for L. pumilum. Tetraploids were obtained by treating with colchicine at 0.02 and 0.04% for 48 hours for L. sargentiae and L. tsingtauense. The optimal oryzalin concentration for the induction of tetraploids was 0.01% for L. tsingtauense and L. sargentiae with 24 or 48 hours treatment, while 0.01% with 24 hours and 0.006% with 48 hours were the optimal oryzalin treatment for L. pumilum. Oryzalin was more efficient than colchicine in inducing tetraploids in the lily species investigated and may be considered as an alternative reagent of colchicine.
Duration of treatment and concentration of reagent both had significant effects on the survival rate, bulblet formation, and chromosome doubling.
Morphological and anatomical characteristics of leaves and stomatal characteristics correlated with ploidy of lily plants.
Chromosome counts and flow cytometry confirmed these findings.
Authors
Sun Ming, Li Xiao-fan, Kong Ying, Shi Jin-fang, Zhang Qi-xiang
Keywords
polyploidy, colchicine, oryzalin, Lilium pumilum, L. sargentiae, L. tsingtauense
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