Articles
SELECTION OF HEAT TOLERANT POTATO LINES VIA IN VITRO SCREENING OF TRUE POTATO SEEDS
Article number
935_32
Pages
225 – 230
Language
English
Abstract
The purpose of this research is to develop an efficient and more rapid in vitro screening method for heat tolerant families while producing microtubers directly from plants grown from true seeds, without need for shoot transplantation.
An in vitro system for early selection of heat tolerant potato was developed on the basis of the tuberization of plants grown from true seeds, without need of transplanting shoots.
This culture technique is less time consuming than traditional in vitro or field selection methods to discriminate between families with differences in heat tolerance and maintains promising clones for adaptation to warm environments as microtubers.
Thirty-nine hybrid families were assessed under three temperature regimes.
At 20 and 25°C, all families produced microtubers in different proportions; while at 30°C the production of microtubers was strongly inhibited.
The mean tuberization rates among seedlings were 10.1, 7.2 and 1.5% at 20, 25 and 30°C, respectively.
The in vitro test results corresponded to the expected heat tolerance of the families.
Further research will reveal the effectiveness of this in vitro technique in respect to field selection.
An in vitro system for early selection of heat tolerant potato was developed on the basis of the tuberization of plants grown from true seeds, without need of transplanting shoots.
This culture technique is less time consuming than traditional in vitro or field selection methods to discriminate between families with differences in heat tolerance and maintains promising clones for adaptation to warm environments as microtubers.
Thirty-nine hybrid families were assessed under three temperature regimes.
At 20 and 25°C, all families produced microtubers in different proportions; while at 30°C the production of microtubers was strongly inhibited.
The mean tuberization rates among seedlings were 10.1, 7.2 and 1.5% at 20, 25 and 30°C, respectively.
The in vitro test results corresponded to the expected heat tolerance of the families.
Further research will reveal the effectiveness of this in vitro technique in respect to field selection.
Authors
Hyunjun Kim, Kwangsoo Cho, Jihong Cho, Youngeun Park , Hyunmook Cho
Keywords
early breeding selection, tissue culture, microtubers
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