Articles
AGROBACTERIUM-MEDIATED TRANSFORMATION OF PLUM CULTIVAR ‘STARTOVAYA’ BY SELF-COMPLEMENTARY HAIRPIN RNA OF PPV-CP GENE
Article number
941_5
Pages
85 – 90
Language
English
Abstract
RNA silencing refers to related processes of post-transcriptional control of gene expression found in plants, animals and fungi.
In higher plants, RNA silencing serves as an adaptive, antiviral defense system, which is transmitted systemically in response to localized virus challenge.
This mechanism is remarkably efficient because, once activated by aberrant or foreign RNA molecules, it is primed against any cytoplasm RNA species sharing sequence homology with the triggering molecules.
Plum (P. domestica L.) leaves of the cultivar Startovaya were transformed using Agrobacterium tumefaciens strain AGL0:pCamPPVRNAi carrying the hygromycin phosphotransferase gene (hpt), the gus gene and self-complementary sequences of fragment of PPV-CP gene separated by an intron to produce a hairpin RNA (hp-RNA) structure.
Regenerated plums on hygromycin media were analyzed by GUS assays and by PCR detection of the PPV CP gene.
In higher plants, RNA silencing serves as an adaptive, antiviral defense system, which is transmitted systemically in response to localized virus challenge.
This mechanism is remarkably efficient because, once activated by aberrant or foreign RNA molecules, it is primed against any cytoplasm RNA species sharing sequence homology with the triggering molecules.
Plum (P. domestica L.) leaves of the cultivar Startovaya were transformed using Agrobacterium tumefaciens strain AGL0:pCamPPVRNAi carrying the hygromycin phosphotransferase gene (hpt), the gus gene and self-complementary sequences of fragment of PPV-CP gene separated by an intron to produce a hairpin RNA (hp-RNA) structure.
Regenerated plums on hygromycin media were analyzed by GUS assays and by PCR detection of the PPV CP gene.
Authors
R.V. Mikhailov , S.V. Dolgov
Keywords
“hairpin” RNA, RNA interference, PPV, transformation, plum, Prunus domestica
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