Articles
GENETIC VARIABILITY IN TEN SPANISH CARDOON POPULATIONS AS ASSESSED BY MORPHOLOGICAL, AGRONOMICAL AND MOLECULAR ANALYSES
Article number
942_13
Pages
115 – 122
Language
English
Abstract
In this study, the genetic diversity of fifty individuals of cardoon, Cynara cardunculus L., from ten Spanish cultivated accessions, was evaluated using morphological traits and inter simple sequence repeat (ISSR) DNA data.
Molecular analysis was carried out using the ISSR technique with thirty primers.
Out of these, eight were polymorphic among the accessions.
A total of 2053 scorable and repeatable DNA fragments were generated, of which 1320 (64.3%) were polymorphic.
In total, 56 ISSR loci were detected with repeatability across 50 cultivars.
The average ISSR locus frequency was 7.0 loci per primer, ranging from 5 to 11 loci per primer.
The data obtained from the different ISSR markers were used to construct a dendrogram on the basis of a similarity genetic distance matrix, using the UPGMA algorithm.
Four quantitative characteristics from the plant and six quantitative and three qualitative characteristics from the leaf stalk were measured.
The most productive varieties were Verde de Peralta, Verde de Calahorra and Lleno de España, with a yield of 76.9, 74.7 and 68.2 t ha-1, respectively.
The least productive cardoon variety was the Rojo de Agreda with 41.9 t ha-1. This work has showed a remarkable level of genetic variation between and within Spanish cardoon cultivars.
All morphological traits were subjected to a Principal Component Analysis in order to visualize the morphological variability of the analysis genotypes and the first axis explains 31.2% of the inertia and it was defined by main rib height, while the second axis explains 25.2% defined by productivity.
Molecular analysis was carried out using the ISSR technique with thirty primers.
Out of these, eight were polymorphic among the accessions.
A total of 2053 scorable and repeatable DNA fragments were generated, of which 1320 (64.3%) were polymorphic.
In total, 56 ISSR loci were detected with repeatability across 50 cultivars.
The average ISSR locus frequency was 7.0 loci per primer, ranging from 5 to 11 loci per primer.
The data obtained from the different ISSR markers were used to construct a dendrogram on the basis of a similarity genetic distance matrix, using the UPGMA algorithm.
Four quantitative characteristics from the plant and six quantitative and three qualitative characteristics from the leaf stalk were measured.
The most productive varieties were Verde de Peralta, Verde de Calahorra and Lleno de España, with a yield of 76.9, 74.7 and 68.2 t ha-1, respectively.
The least productive cardoon variety was the Rojo de Agreda with 41.9 t ha-1. This work has showed a remarkable level of genetic variation between and within Spanish cardoon cultivars.
All morphological traits were subjected to a Principal Component Analysis in order to visualize the morphological variability of the analysis genotypes and the first axis explains 31.2% of the inertia and it was defined by main rib height, while the second axis explains 25.2% defined by productivity.
Authors
D. Migliaro, P. Gómez di Marco, A. Esteban, J.A. Fernández, J.I. Macua, I. Lahoz, D. Niñirola, C. Egea-Gilabert
Keywords
Cynara cardunculus, ISSR markers, genetic diversity, agronomical traits
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