Articles
BACTERIOPHAGE LAMBDA GENOMIC LIBRARY CONSTRUCTION OF HYLOCEREUS POLYRHIZUS AND HYLOCEREUS UNDATUS
Article number
943_5
Pages
65 – 71
Language
English
Abstract
Genomic library construction for the two species of dragon fruits, Hylocereus undatus and Hylocereus polyrhizus, using bacteriophage Lambda Fix II with 20 kb inserts was reported.
About 80-100 plaques were observed for each plate, indicating that bacteriophage has lysed the host cells, XL1-Blue MRA (P2). Best methods for genomic library construction were discussed in details.
These include total DNA digestion using restriction enzyme, BamHI, phenol-chloroform purification of the concentrated insert DNA, ligation, packaging, and titering the packaging reaction.
About 80-100 plaques were observed for each plate, indicating that bacteriophage has lysed the host cells, XL1-Blue MRA (P2). Best methods for genomic library construction were discussed in details.
These include total DNA digestion using restriction enzyme, BamHI, phenol-chloroform purification of the concentrated insert DNA, ligation, packaging, and titering the packaging reaction.
Authors
Z.X. Loo, L.K.P. Daniel, S. Chandran
Keywords
Hylocereus undatus, Hylocereus polyrhizus, genomic library, lambda vector, partial fill-in, titer
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